Androgen Action (eBook, PDF)

Methods and Protocols
Redaktion: Saatcioglu, Fahri

• Format: PDF

Produktbeschreibung

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 389
• Erscheinungstermin: 9. August 2011
• Englisch
• ISBN-13: 9781617792434
• Artikelnr.: 64701305

Inhaltsangabe

Molecular Mechanisms of Androgen Action - A Historical Perspective.- Androgen Action during Prostate Carcinogenesis.- Androgen Quantitation in Prostate Cancer Tissue using Liquid Chromatography Tandem Mass Spectrometry.- Ligand Competition Binding Assay for the Androgen Receptor.- Analysis of Androgen Receptor Activity by Reporter Gene Assays.- Identification and Characterization of Androgen Response Elements.- In vitro and In vivo Silencing of the Androgen Receptor.- Analysis of Interdomain Interactions of the Androgen Receptor.- Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells.- FRET Analysis of Androgen Receptor Structure and Biochemistry in Living Cells.- Analysis of Nuclear Receptor Acetylation.- Analysis of Androgen Receptor SUMOylation.- Analysis of Ligand-specific Co-repressor Binding to the Androgen Receptor.- Detection of Ligand-selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF.- Global Identification of Androgen Response Elements.- Tissue Specific Knockout of Androgen Receptor in Mice.- Methodology to Investigate Androgen-sensitive and Castration-resistant Human Prostate Cancer Xenografts in Preclinical Setting.- Automated Microscopy and Image Analysis for Androgen Receptor Function.- Androgen Regulation of ETS-gene Fusion Transcripts in Prostate Cancer.- Regulation of Apoptosis by Androgens in Prostate Cancer Cells.- Analysis of Androgen Receptor Rapid Actions in Cellular Signaling Pathways: Receptor /Src Association.- Analysis of Androgen-induced Increase in Lipid Accumulation in Prostate Cancer Cells.

Rezensionen

From the reviews:

"The Editor asked more than 50 experts from three continents and put them together to contribute with their up-to-date written chapters. This valuable volume begins with two chapters on the history of androgen research, especially with androgen action and prostate carcinogenesis. ... This volume will certainly provide the reader with a comprehensive overview of, and practical guidance on, the discrete methodologies which are propelling androgen research forward." (Endocrine Regulations, October, 2012)

"This book details protocols to determine androgen levels, actions mediated by AR, and cross-talk with other key signaling networks. ... The techniques are most appropriate for biochemists and molecular and cellular biologists who are engaged in the study of androgen-mediated events. ... This effectively written and thought-out book will serve as useful resource to decipher the complex actions of androgens. ... The compilation of knowledge and wisdom in this book will be beneficial to current and future researchers." (Clay E. S. Comstock, Doody's Book Reviews, February, 2012)