Roger L. Lundblad

Approaches to the Conformational Analysis of Biopharmaceuticals (eBook, PDF)

• Format: PDF

Produktbeschreibung

Conformational analysis is becoming more critical in the development of generic biopharmaceuticals. This book describes methods used to establish comparability between potentially identical products, providing researchers with a critical description of studies drawn from the current literature. The text covers physical methods, such as differential scanning calorimetry, light scanning, and analytical ultracentrifugation, as well as chemical methods, including hydrogen-deuterium exchange and trace labeling. The author shows how these methods support comparability claims for generic biologicals and assure structural integrity of active pharmaceutical ingredients in final drug products.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Taylor & Francis
• Seitenzahl: 366
• Erscheinungstermin: 15. Dezember 2009
• Englisch
• ISBN-13: 9781439807811
• Artikelnr.: 57534219

Autorenporträt

After postdoctoral work at Rockefeller University, New York, Dr. Roger L. Lundblad joined the faculty of the University of North Carolina at Chapel Hill in 1968. He joined the Hyland division of Baxter Healthcare in 1990. Currently, he is an independent consultant and biotechnology writer based in Chapel Hill, North Carolina. He is an adjunct professor of pathology at the University of North Carolina at Chapel Hill and an editor in chief of the Internet Journal of Genomics and Proteomics.

Inhaltsangabe

Introduction to Biopharmaceutical Conformational Analysis: Issues and
Methods. Comparability of Biotechnological/Biological Products and
Biological Generics. Application of Native Electrophoresis for the Study of
Protein Conformation. Affinity Chromatography Including Hydrophobic
Interaction Chromatography in the Study of Biopolymer Conformation.
Size-Exclusion Chromatography and Biomolecular Conformation. Use of
Analytical Ultracentrifugation to Study Biomolecular Conformation. Use of
Differential Scanning Calorimetry to Measure Conformational Change in
Proteins and Other Biomacromolecules. Light Scattering and
Biomacromolecular Conformation. Use of Luminescence to Measure
Conformational Change in Biopharmaceuticals with Emphasis on Protein and
Protein Drug Products. Near-Infrared Spectroscopy and Macromolecular
Conformation. The Use of Mid-Infrared and Fourier Transform Infrared
Spectroscopy to Study Conformation of Biomacromolecules. Use of Raman
Spectroscopy to Evaluate Biopharmaceutical Conformation. Use of UV-VIS
Spectrophotometry for the Characterization of Biopharmaceutical Products.
Use of Optical Rotatory Dispersion and Circular Dichroism to Study
Therapeutic Biomacromolecule Conformation. Use of Nuclear Magnetic
Resonance for the Characterization of Biotherapeutic Products. Use of
Chemical Probes for the Study of Protein Conformation. Use of Hydrogen
Exchange in the Study of Biopharmaceutical Conformation. Use of Chemical
Modifi cation for the Conformational Analysis of Biopharmaceuticals. Use of
Immunology to Characterize Biopharmaceutical Conformation. Use of Limited
Proteolysis to Study the Conformation of Proteins of Biotechnology
Interest. Other Technologies for the Characterization of Conformational
Change in Biopharmaceuticals. Development of an Experimental Approach for
the Study of the Conformation of a Biological Therapeutic Product.