Auditory and Vestibular Research (eBook, PDF)

Methods and Protocols
Redaktion: Sokolowski, Bernd

• Format: PDF

Produktbeschreibung

While early studies of the auditory/vestibular sciences provided insights into the anatomy and neurophysiology of these systems and produced a prosthetic cochlear implant, the rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order to best provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Auditory and Vestibular Research: Methods and Protocols is an ideal guide to the field and a useful tool for exploring genes and proteins in other systems as well, especially systems in which tissues are scarce and a comparative approach lends itself to discovering the underlying causes of human disorders.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 428
• Erscheinungstermin: 8. Oktober 2008
• Englisch
• ISBN-13: 9781597455237
• Artikelnr.: 64615687

Inhaltsangabe

Nucleic Acid Protocols.- RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning.- Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays.- In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections.- Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification.- Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus.- The Practical Use of Cre and loxP Technologies in Mouse Auditory Research.- Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium.- Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear.- Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear.- Molecular Biology of Vestibular Schwannomas.- Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens.- Fluorescence "In Situ" Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa.- Positional Cloning of Deafness Genes.- Amino Acid Protocols.- Twist-Off Purification of Hair Bundles.- Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System.- The Use of 2-D Gels to Identify Novel Protein-Protein Interactions in the Cochlea.- Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis.- In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation.- Identification of Transcription Factor-DNA Interactions Using Chromatin Immunoprecipitation Assays.- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins inInner-Ear Sensory Epithelia.- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research.- Imaging Protocols.- Fluorescence Microscopy Methods in the Study of Protein Structure and Function.- Ion Imaging in the Cochlear Hair Cells.- Atomic Force Microscopy in Studies of the Cochlea.