B Cell Protocols (eBook, PDF)

Redaktion: Gu, Hua; Rajewsky, Klaus

• Format: PDF

Produktbeschreibung

Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 292
• Erscheinungstermin: 4. Februar 2008
• Englisch
• ISBN-13: 9781592597963
• Artikelnr.: 64692500

Inhaltsangabe

Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen.- Characterization of B-Cell Maturation in the Peripheral Immune System.- Identification of B-Cell Subsets.- Analysis of B-Cell Life-Span and Homeostasis.- Differentiation of B Lymphocytes From Hematopoietic Stem Cells.- Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System.- Conditional Gene Mutagenesis in B-Lineage Cells.- Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells.- T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences.- of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors.- Immunoglobulin Class Switching.- In Vitro and In Vivo Assays of B-Lymphocyte Migration.- Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo.- B-Cell Signal Transduction.- Isolation of Lipid Rafts From B Lymphocytes.- Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells.- Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice.- Analysis of B-Cell Signaling Using DT40 B-Cell Line.- B-Cell Development and Pre-B-1 Cell Plasticity In Vitro.