Basic Cloning Procedures (eBook, PDF)

Redaktion: Berzins, Valdis

• Format: PDF

Produktbeschreibung

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

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Produktdetails

• Verlag: Springer Berlin Heidelberg
• Seitenzahl: 169
• Erscheinungstermin: 6. Dezember 2012
• Englisch
• ISBN-13: 9783642719653
• Artikelnr.: 53097444

Inhaltsangabe

1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.