This book is comprised of seven chapters and begins with a discussion on chemical fixation, with particular reference to fixatives and the hazards, precautions, and safe handling of reagents, as well as the preparation of buffers and tissue blocks. The reader is then introduced to the standard procedure for fixation, rinsing, dehydration, and embedding. Subsequent chapters focus on sectioning, cryofixation, and cryoultramicrotomy; positive and negative staining; and the use of support films. The final chapter presents a wide variety of specimens such as algae, amoeba, anthers, actin filaments, bacteria, and cells in culture.
This monograph is essentially a laboratory handbook intended for students, technicians, teachers, and research scientists in biology and medicine.
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