Biophysics and the Challenges of Emerging Threats (eBook, PDF)

Redaktion: Puglisi, Joseph

• Format: PDF

Produktbeschreibung

Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1-3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4-6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11-13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16-18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Springer Netherlands
• Seitenzahl: 179
• Erscheinungstermin: 25. Mai 2009
• Englisch
• ISBN-13: 9789048123681
• Artikelnr.: 44219619

Inhaltsangabe

A Simple Model for Protein Folding.- Complementarity of Hydrophobic/Hydrophilic Properties In Protein-Ligand Complexes: A New Tool to Improve Docking Results.- Structures of Cvnh Family Lectins.- Biophysical Approaches To Study Dna Base Flipping.- The Diversity of Nuclear Magnetic Resonance Spectroscopy.- Improved Dye Stability in Single-Molecule Fluorescence Experiments.- The Evaluation of Isotope Editing and Filtering for Protein-Ligand Interaction Elucidation by Nmr.- Ribosome: an Ancient Cellular Nano-Machine for Genetic Code Translation.