Biotechnology (eBook, ePUB)

A Laboratory Course
Redaktion: Becker, Jeffrey M.; Zachgo, Eve Ann; Caldwell, Guy A.

• Format: ePub

Produktbeschreibung

The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences.

• All exercises and appendixes have been updated
• Includes new exercises on:
• Polymerase chain reaction
• Beta-Galactosidase detection in yeast colonies
• Western blotting
• New procedures introduced for:
• Large-scale plasmid isolation
• Yeast transformation
• DNA quantitation
• New appendixes added, one of which provides details on accessing biological information sites on the Internet (World Wide Web)
• Use of non-radioactive materials and easy access to microbial cultures
• Laboratory exercises student tested for seven years

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 261
• Erscheinungstermin: 11. März 1996
• Englisch
• ISBN-13: 9780080528199
• Artikelnr.: 47460211

Inhaltsangabe

Suggested Schedule for Laboratory Exercises.Introductory Notes.Record
Keeping and Safety Rules.Format of Student Laboratory Records.The Ten
Commandments of Record Keeping.Safety Rules in the Laboratory.Exercise 1:
Aseptic Culture and Establishing Pure Cultures.Exercise 2: Preparation of
Culture Media.Exercise 3: The Growth Curve.Exercise 4: Isolation of Plasmid
DNA from Escherichia coli: The Mini-Prep.Exercise 5: Purification,
Concentration, and Quantitation of DNA.Exercise 6: Large-Scale Isolation of
Plasmid DNA by Column Chromatography.Exercise 7: Amplification of a lacZ
.Gene Fragment by the Polymerase Chain Reaction.Exercise 8: Restriction
Digestion and Agarose Gel Electrophoresis.Exercise 9: Southern
Transfer.Exercise 10: Preparation, Purification, and Hybridization of
Probe.Exercise 11: Transformation of Saccharomyces cerevisiae.Exercise 12:
Isolation of Plasmid from Yeast and Escherichia coli
Transformation.Exercise 13: Protein Assays.Exercise 14: Qualitative Assay
for Galactosidase in Yeast Colonies.Exercise 15: Determination of
Galactosidase in Permeabilized Yeast Cells.Exercise 16: Assay of
Galactosidase in Cell Extracts.Exercise 17: Galactosidase
Purification.Exercise 18: Western Blot: Probe of Protein Blot with Antibody
to Galactosidase.Appendix 1: Alternate Protocols and Experiments:Exercise
1A: Isolation and Characterization of Auxotrophic Yeast Mutants.Exercise
2A: Measurement of pH.Exercise 3A: Use of the Spectrophotometer.Exercise
6A: Isolation of Plasmid DNA: The Maxi-Prep.Exercise 10A: Colony
Hybridization.Appendix 2: Buffer Solutions.Appendix 3: Preparation of
Buffers and Solutions.Appendix 4: Properties of Some Common Concentrated
Acids and Bases.Appendix 5: Use of Micropipettors.Appendix 6: Safe Handling
of Microorganisms. Appendix 7: List of Cultures.Appendix 8: Storage of
Cultures and DNA.Appendix 9: Sterilization Methods.Appendix 10: Preparation
of Stock Solutions for Culture Media.Appendix 11: Growth in Liquid
Medium.Appendix 12: Determination of Viable Cells.Appendix 13:
Determination of Cell Mass.Appendix 14: Determination of Cell
Number.Appendix 15: Nomenclature of Strains.Appendix 16: Glassware and
Plasticware.Appendix 17: Preparation of Tris and EDTA.Appendix 18: Basic
Rules for Handling Enzymes.Appendix 19: Effects of Common Contaminants on
Protein Assays.Appendix 20: Manufacturers' and Distributors'
Addresses.Appendix 21: Surfing the Bionet: World Wide Web
Addresses.Glossary.Subject Index.