Cardiac Cell and Gene Transfer (eBook, PDF)

Redaktion: Metzger, Joseph M.

• Format: PDF

Produktbeschreibung

Cellular and gene-based experimental techniques promise powerful new therapeutic agents for the remediation of both acquired and inherited cardiovascular disease. In Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications, Joseph M. Metzger and a panel of leading scientific experts detail many novel techniques and strategies for the cellular and genetic modification of heart function. These highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro. Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today's unanswered questions about heart disease and ultimately to improve the heart performance in all their patients.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 253
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781592593507
• Artikelnr.: 64700293

Inhaltsangabe

Vectors for Cardiac Gene Transfer.- Adenoviral Vectors.- Gutted Adenoviral Vectors for Gene Transfer to Muscle.- Dual Vector Expansion of the Recombinant AAV Packaging Capacity.- Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes.- Cell Transfer to Myocardium.- Cell Therapy in the Heart.- Cardiac Cell Transplantation.- Cell Grafting for Cardiac Repair.- Vectors for Cardiac Gene Transfer.- Suppressor tRNAs.- Antisense Oligonucleotides.- Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants.- Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes.- Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes.- In Vivo Cardiac Gene Transfer.- Embryonic and Neonatal Cardiac Gene Transfer In Vivo.- Efficient Viral Gene Transfer to Rodent Hearts In Vivo.- Direct Gene Transfer to the Adult Rodent Myocardium In Vivo.- Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures.- Coronary Perfusion Cocktails for In Vivo Gene Transfer.- Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of ?-Adrenergic Receptor Signaling Components.- Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo.

Rezensionen

"The book is surprisingly up-to-date...it is quite unique, as there is no similar compendium of detailed protocols. I found the book to be highly readable. It is a particularly useful reference work for cardiovascular scientists who are already using gene transfer approaches in their research. It is also a great starting place for cardiovascular investigators who are just beginning to use these methods in their labs." -Doody's Health Sciences Book Review Journal