Cardiac Gene Expression (eBook, PDF)

Methods and Protocols
Redaktion: Zhang, Jun; Rokosh, Gregg

• Format: PDF

Produktbeschreibung

This book presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. The concluding section of the book highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 366
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781597450300
• Artikelnr.: 64701066

Inhaltsangabe

Cardiac Gene Expression.- Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells.- Expression Profiling Using Affymetrix GeneChip® Probe Arrays.- Serial Analysis of Gene Expression (SAGE).- Functional Genomics by cDNA Subtractive Hybridization.- Statistical Methods in Cardiac Gene Expression Profiling.- Cardiac Gene Regulation.- Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR).- Quantitative (Real-Time) RT-PCR in Cardiovascular Research.- RNase Protection Assay for Quantifying Gene Expression Levels.- In Situ Hybridization.- Cardiac Gene Regulation.- Characterization of cis-Regulatory Elements and Transcription Factor Binding.- Mapping Transcriptional Start Sites and In Silico DNA Footprinting.- Characterization of Cardiac Gene Promoter Activity.- In Silico Assessment of Regulatory cis-Elements and Gene Regulation.- Comparative Genomics.- Developing Computational Resources in Cardiac Gene Expression.- Cardiac Single Nucleotide Polymorphisms.- In Silico Analysis of SNPs and Other High-Throughput Data.- Discovery and Identification of Sequence Polymorphisms and Mutations with MALDI-TOF MS.- Gene Overexpression and Targeting in the Myocardium.- Conditional Targeting.- Cardiomyocyte Preparation, Culture, and Gene Transfer.- Adeno-Associated Viral Vector-Delivered Hypoxia-Inducible Gene Expression in Ischemic Hearts.- Lentivirus-Mediated Gene Expression.

Rezensionen

"...a timely, detailed and well edited methodss text which would be a valuable addition to any cardiovascular research laboratory." -Molecular Biotechnology