Cardiovascular Proteomics (eBook, PDF)

Methods and Protocols
Redaktion: Vivanco, Fernando

• Format: PDF

Produktbeschreibung

This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 432
• Erscheinungstermin: 5. Februar 2008
• Englisch
• ISBN-13: 9781597452144
• Artikelnr.: 64692354

Inhaltsangabe

Analysis of the Heart Proteome.- Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics.- Analyzing the Cardiac Muscle Proteome by Liquid Chromatography-Mass Spectrometry-Based Expression Proteomics.- Two-Dimensional Differential Gel Electrophoresis of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion.- Analysis of Antihypertensive Drugs in the Heart of Animal Models.- A Solubility Optimization Protocol for Two-Dimensional Gel Electrophoresis of Cardiac Tissue.- A Method for the Effective Depletion of Albumin From Cellular Extracts.- Heart Subproteomes.- Subcellular Fractionation.- Subfractionation of Heart Tissue.- Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and SELDI-TOF.- Proteomic Analysis of the Subunit Composition of Complex I (NADH:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria.- Identification of Targets of Phosphorylation in Heart Mitochondria.- Proteomics of Human Atheroma Plaques.- Characterization of the Human Atheroma Plaque Secretome by Proteomic Analysis.- Characterization of HSP27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome.- Western Array Analysis of Human Atherosclerotic Plaques.- The Proteome of Cells of the Cardiovascular System.- The Proteome of Endothelial Cells.- Proteomic Study of Caveolae and Rafts Isolated From Human Endothelial Cells.- Proteomic Identification of S-Nitrosylated Proteins in Endothelial Cells.- The Proteome and Secretome of Human Arterial Smooth Muscle Cell.- Real-Time In Vivo Proteomic Identification of Novel Kinase Substrates in Smooth Muscle.- Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain.- Isolation and Culture of Adult Mouse Cardiac Myocytes.- Proteomic Analysis of Foam Cells.- Isolation of the Platelet Releasate.- Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation.- Characterization of Circulating Human Monocytes by Proteomic Analysis.- Cardiovascular Biomarkers in Plasma.- Cardiovascular Biomarkers Discovery by SELDI-TOF Mass Spectrometry.- Detection of Biomarkers of Stroke Using SELDI-TOF.- Depletion of High-Abundance Proteins in Plasma by Immunoaffinity Substraction for Two-Dimensional Difference Gel Electrophoresis Analysis.- A Rapid, Economical, and Reproducible Method for Human Serum Delipidation and Albumin and IgG Removal for Proteomic Analysis.- Bioinformatics: Emerging Technologies.- Mitoproteome.- Techniques to Decipher Molecular Diversity by Phage Display.

Rezensionen

"...a fine collection of methods and protocols currently employed in cardiovascular proteomics that will be of great use to all who are invovled in cardiovascular research." -Proteomics Clin. Appl. "...a thorough set of protocols for the cardiac biology community. Most laboratories will have some of these in place, but can profit from the broad coverage of this book." -Doody's Book Review, Weighted Numerical Score: 76 - 3 stars