Cell Cycle Checkpoint Control Protocols (eBook, PDF)

Redaktion: Lieberman, Howard B.

• Format: PDF

Produktbeschreibung

In recent years, cell cycle checkpoints, cellular mechanisms that control the cell cycle and ensure genomic stability, have emerged as significant factors in carcinogenesis and in cancer cells. In Cell Cycle Checkpoint Control Protocols, leading investigators present their best methodologies to probe the mechanisms underlying cell cycle regulation and checkpoint control. Using mammalian, yeast, and frog systems, these experts describe readily reproducible methods to induce cell cycle checkpoints, detect changes in cell cycle progression, identify and analyze genes and proteins that regulate the process, and characterize chromosomal status as a function of cell cycle phase and progression. Each fully tested technique includes step-by-step instructions written by an investigator who performs it frequently, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Taken as a whole, the collection describes the major methodologies used by researchers in the field. Cutting-edge and highly practical, Cell Cycle Checkpoint Control Protocols provides an extensive array of detailed protocols by which both experienced and novice investigators may successfully illuminate questions concerning cell cycle control.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 376
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592596461
• Artikelnr.: 64700501

Inhaltsangabe

"Part I. Induction and Detection of Changes in Cell Cycle Progression

Methods to Induce Cell Cycle Checkpoints
Howard B. Lieberman and Kevin M. Hopkins

Methods for Synchronizing Mammalian Cells
Michael H. Fox

Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation
Tej K. Pandita

Analysis of the Mammalian Cell Cycle by Flow Cytometry
Haiying Hang and Michael H. Fox

Methods for Detecting Cells in S Phase
Wei-Hsin Sun and Melvin L. DePamphilis

Yeast Cell Synchronization
Audra Day, Colette Schneider, and Brandt L. Schneider

Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow Cytometry
Hong Zhang and Wolfram Siede

Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle
Eliana B. Gómez and Susan L. Forsburg

Part II. Analysis of Genes Involved in Checkpoint Control

Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes
Scott Davey

Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
Sally A. Amundson and Albert J. Fornace, Jr.

Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens
Jian Zhang, Lisa Ottmers, and Brandt L. Schneider

Gene Targeting in Cultured Human Cells
Todd A. Waldman

Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene
Migdalisel Colòn and Nancy C. Walworth

In Vitro Mutagenesis to Define Functional Domains
Jian Qin, Zhe Peng, and Maureen V. McLeod

Use of Gene Overexpression to Assess Function in Cell Cycle Control
Erik K. Flemington and Antonio Rodriguez

Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
Chenguang Wang, Maofu Fu, and Richard G. Pestell

Part III. Analysis of Proteins "

Rezensionen

"...includes step-by-step instructions written by an investigator who performs it frequently, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls." -- Tumori