Richard J. Meyer, Stacie A. Brown

Challenges of the Unseen World (eBook, PDF)

A Laboratory Course in Microbiology

• Format: PDF

Produktbeschreibung

Solving real-world health challenges in a learning environment You are at an exciting gateway into the world of microorganisms. With nothing more than basic lab equipment such as microscopes, Petri dishes, media, and a handful of reagents, you will learn to isolate, grow, and identify bacteria that live all around us. This is no ordinary microbiology laboratory course; not only will you learn how to streak plates, use a microscope, perform a Gram stain, and prepare serial dilutions and spread plates--fundamental skills found in every microbiologist's toolkit--you will solve a series of public health-related challenges that many professional microbiologists encounter in their work. By the end of this course, you will: * Determine the origin of a nosocomial infection. Using foundational and molecular methods, you will determine whether the infections occurring in hospitalized patients are the result of contaminated medical items. * Select the antibiotic to treat a patient with Crohn's disease. You will find minimum inhibitory concentrations of various antibiotics for a Pseudomonas strain associated with Crohn's disease. * Pinpoint the source of lettuce contaminated with E. coli. Using molecular tools you will investigate a common food safety challenge, antibiotic-resistant E. coli and the potential for spread of this resistance in the environment. * Find the farm releasing pathogens into a stream used for drinking water. Using bacteriophage load in water samples, you will locate the source of fecal contamination in the water supply of a village in an underdeveloped country. * Evaluate the potential of bacteria to cause a urinary tract infection. You will test for biofilms, quorum sensing behavior, and chemotaxis and assess which disinfectants would be most effective for sanitizing contaminated surfaces. Microbiology educators and researchers Richard Meyer and Stacie Brown have created this hands-on, engaging introduction to the essential laboratory skills in the microbial sciences that is sure to change the way you view the world around you.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 204
• Erscheinungstermin: 6. August 2020
• Englisch
• ISBN-13: 9781555819958
• Artikelnr.: 59981442

Autorenporträt

Richard J. Meyer, Ph.D., is a professor in the Department of Molecular Biosciences at the University of Texas at Austin. He joined the Department of Microbiology in the University of Texas at Austin in 1978, and has been at that institution ever since. From the beginning of his career, Meyer has been interested in the hands-on aspect of teaching biology to undergraduates. He developed the introductory microbiology laboratory course currently used at the University of Texas at Austin. It was the pedagogical approaches that are used in this course that inspired him to develop the manual you hold in your hands. Over more than forty years, Meyer's research was on the molecular mechanisms of replication and conjugative transfer of broad host-range plasmids. Stacie A. Brown, Ph.D., is director of first year biology laboratories and a member of the biology department at Southwestern University. Prior to her current position, she taught microbiology courses for biology majors and pre-nursing students while also overseeing the microbiology labs, at Texas State University. For several years, she also taught microbiology labs and courses at the University of Texas at Austin. Her experience teaching microbiology labs to thousands of undergraduates ensures that the challenge-based microbiology labs in this manual will work in any introductory laboratory course in undergraduate microbiology.

Inhaltsangabe

Preface

About the Authors

Introduction

The scientific method

Experimental design

Big data

Documentation

Safety

Student Laboratory Safety Contract

Appendix

Challenge One: Identifying the bacteria causing infections in hospital patients

Lab One

Background

Diversity and pure cultures

Bright field and phase contrast microscopy

Learning outcomes

Objectives

Part 1: Isolate bacteria from a mixed culture: Procedure: Streaking for isolated colonies

Part 2: Examine bacterial cells under the microscope

Procedure: Making a wet mount

Procedure: Using the microscope

Preparation for next lab

Questions

Lab Two

Background

Colony morphology and optimum temperature for growth

Cell shape and bacterial spores

The cell envelope

Learning outcomes

Part 1: Describe the colony morphology of the unknown

Part 2: Describe the characteristics of an individual cell viewed under themicroscope

Part 3: Determine the optimum temperature for growth

Part 4: Determine if the unidentified microorganism is Gram-positive or Gram-negative.

Procedure: Doing a Gram stain

Preparation for next lab

Questions

Lab Three

Background

Modes of energy generation in bacteria

Learning outcomes

Part 1: Can the unidentified microorganism grow in the presence of bile salts and ferment lactose?

Procedure: Streaking cells on MacConkey-lactose plates

Part 2: Can the unidentified microorganism ferment glucose?

Procedure: Glucose fermentation test

Part 3: Does the unidentified microorganism use cytochrome C duringrespiration (Gram-negative bacteria)?

Procedure: Oxidase test

Part 4: Does the microorganism make catalase (Gram-positive bacteria)?

Procedure: Catalase test

Part 5: Is the microorganism motile?

Procedure: Soft agar motility assay

Questions

Solving Challenge One

Preparing for Challenge Two

Questions

Bibliography

Challenge Two: Confirming the identification of a microorganism by sequencing the 16S rRNA gene

Questions before you begin the challenge

Lab One: Background

Classification of bacteria and 16S rRNA gene

Polymerase chain reaction (PCR)

Lab One: Learning outcomes

Lab One: Objective

Part 1: Obtain enough DNA for sequencing: amplify the 16S rRNA gene by PCR

Procedure:Diluting from stock solutions:

Lab One: Questions

Lab Two: Background

Agarose gel electrophoresis

Dideoxy DNA sequencing

Lab Two: Learning outcomes

Lab Two: Objectives

Part 1: Visualize the PCR product by agarose gel electrophoresis

Procedure:Making an agarose gel and carrying out gel electrophoresis

Part 2: Submit sample for DNA sequencing

Lab Two: Questions

Solving Challenge Two: Background

Solving Challenge Two: Learning outcomes

Solving Challenge Two: Objective

Identifying the unknown microorganism from the 16S rRNA gene sequence

Procedure: Preparing the sequence for analysis

Procedure: Doing a BLAST search

Questions

Bibliography

Challenge Three: Choosing an antibiotic to alleviate the symptoms of Crohn's disease

Questions before you begin the challenge