Cryopreservation and Freeze-Drying Protocols (eBook, PDF)

Redaktion: Day, John G.; Stacey, Glyn

• Format: PDF

Produktbeschreibung

This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.

The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 347
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781597453622
• Artikelnr.: 64700361

Autorenporträt

John G. Day, Scottish Association for Marine Science, Argyll, UK / Glyn Stacey, NIBSC, South Mimms, UK

Inhaltsangabe

Long-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers.- The Principles of Freeze-Drying.- Principles of Cryopreservation.- Lyophilization of Proteins.- Vacuum-Drying and Cryopreservation of Prokaryotes.- Freeze-Drying of Yeast Cultures.- Cryopreservation of Yeast Cultures.- Freeze-Drying Fungi Using a Shelf-Freeze Drier.- Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying.- Cryopreservation of Microalgae and Cyanobacteria.- Cryopreservation of Plant Cell Suspensions.- Cryopreservation of Shoot Tips and Meristems.- Cryopreservation of Desiccation-Tolerant Seeds.- Cryopreservation of Fish Sperm.- Cryopreservation of Avian Spermatozoa.- Cryopreservation of Animal and Human Cell Lines.- Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use.- Cryopreservation of Human Embryonic Stem Cell Lines.- Cryopreservation of Primary Animal Cell Cultures.- Cryopreservation of Red Blood Cells and Platelets.- Cryopreservation of Mammalian Semen.- Cryopreservation of Mammalian Oocytes.- Cryopreservation of Mammalian Embryos.

Rezensionen

From the reviews of the second edition:

"...practical and reliable resource for the conservation and sustainable use of biological resources."-Anticancer Research

"This book is a welcome addition to the relatively sparse comprehensive reference publications on preservation methodologies which are currently available. It begins sensibly with a grounding in the principles involved in preservation, in logical and easy to follow steps. It is well laid out and covers a wide spectrum of cell/organism types in the one volume ... ." (Peter Green, Molecular Biotechnology, December, 2007)