Cytoskeleton Methods and Protocols (eBook, PDF)

Redaktion: Gavin, Ray H.

• Format: PDF

Produktbeschreibung

In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls.

Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 391
• Erscheinungstermin: 18. September 2009
• Englisch
• ISBN-13: 9781607613763
• Artikelnr.: 69699187

Inhaltsangabe

Live-Cell Imaging of the Cytoskeleton.- High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis.- Imaging the Cytoskeleton in Live Xenopus laevis Embryos.- Live-Cell Imaging of the Cytoskeleton and Mitochondrial-Cytoskeletal Interactions in Budding Yeast.- Dynamics of Actin Filaments and Microtubules.- Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization.- Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts.- Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components.- Quantitative Fluorescence Microscopy Techniques.- A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule.- Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots.- Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells.- Electron Microscopy Protocols for Imaging the Cytoskeleton.- Imaging Cytoskeleton Components by Electron Microscopy.- Purification and Localization of Intraflagellar Transport Particles and Polypeptides.- High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies.- Strategies for Imaging Microtubules in Plant Cells.- Analysis of Cell and Organelle Motility.- A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity.- Melanosome Motility in Fish Retinal Pigment Epithelial Cells.- Analysis of Properties of Cilia Using Tetrahymena thermophila.- Application of Cell Traction Force Microscopy for Cell Biology Research.- 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics.- Detergent-Extracted Models for the Study of Cilia or Flagella.- Geneticand Proteomics Approaches for Cytoskeleton Protein Function.- The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions.- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.- Proteomic Tools for the Analysis of Cytoskeleton Proteins.

Rezensionen

From the reviews of the second edition:

"In this second edition it is particularly stressed the cytoskeleton study at the different levels of life organization ... . chapters incorporate a clear introduction, a useful lists of the necessary materials and reagents and the usual (so appreciated by beginners) step-by-step laboratory protocols to avoid the troubleshooting and pitfalls of the bench journey. Cell and developmental biologists are the primary targets as an audience ... ." (Carlo Alberto Redi, European Journal of Histochemistry, Spring, 2010)

"The book contains an interesting mix of chapters some of which cover basic concepts and ideas that are easily transferrable to other systems and others that are more specialised. ... overall the book contains many useful nuggets of information and will be very useful for anyone wanting to try out a new technique or approach covered by the various chapters." (Michelle Peckham, Infocus Magazine, Issue 21, March, 2011)

"This second edition of Cytoskeleton Methods and Protocols ... has the chapters written by different authors. Those authors which have highly experienced and are widely recognized as experts in their fields show in this book detailed protocols combining biochemistry, immunology, genetics, microscopy, and image analysis with integrative approaches for investigations of cytoskeleton components. Each protocol is provided with step-by-step instructions and informative notes that lead both novice and experienced investigators to a successful use. ... serve as an ideal guide to different kind of investigators." (Ricardo Ambrósio Fock, Brazilian Journal of Pharmaceutical Sciences, Vol. 47 (2), Summer, 2011)