Carrying on the high standards of the much respected first edition, Diagnostic Bacteriology Protocols, Second Edition, has been fully updated to include the novel identification, detection, and typing technologies that are transforming the field of diagnostic testing. The authors, working at the forefront of diagnostic test development, highlight these new technologies and describe current and future molecular diagnostic tests and related nucleic acid extraction methods. The protocols range from advanced molecular detection, quantification, and typing systems, to protocols for diagnostic protein identification, serological testing, and cell culture-based assays. The emphasis is on nucleic acid-based diagnostics and alternative biochemically and immunologically based formats that can provide significant potential for multiparameter testing and automation. Several protocols describe specific organisms that may be easily modified to detect other species of interest. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Forward looking and highly practical, Diagnostic Bacteriology Protocols, Second Edition illuminates the cutting-edge technologies now available for bacterial pathogen detection and identification and demonstrates their power and ease of use in a wide variety of applications.
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From the reviews:
"The text is lucid and wonderfully brief...The techniques described have universal application to bacteriological analyses regardless of one's special interests or focus...a well and necessary addendum for all laboratories engaged in or planning to perform bacterial identifications..." -Quarterly Review of Biology
"...a useful addition to the portfolio of bioscience laboratories" -Microbiology Today
"A very useful book for the bench scientist interested in techniques for differentiating bacterial isolates...should prove useful in a wide range of industrial and academic applications." -Doody's Health Sciences Book Review Journal
"...undoubtedly a useful addition to the series and would be of use to research scientists." -Journal of Clinical Pathology
From the reviews of the second edition:
"This concise volume contains simple introductions to many techniques to supplement the traditional agar plate ... . The text is easy and clear to follow ... . the aim of the book is to provide ideas. The chapters can, therefore, be read for inspiration ... . Step-by-step guide to many of the procedures are included, so those about to embark on a new technique, or even those simply wanting to understand the principles ... will be able to benefit. ... A handy reference ... ." (Dr. J Cargill, acp News, Winter, 2006)
"This very practical volume provides concise, state of the art molecular diagnostic bacteriology protocols for a range of species ... . In my view this book provides a valuable additional source of applied protocols for scientists interested in the detection and differentiation of bacteria ... . I strongly recommend this book for purchase by laboratory-based scientists interested in bacterial detection and differentiation." (David C. Coleman, SGM- Society for General Microbiology, 2007)
"The hard-cover publication in A5 format amounting to 225 pages including a six-page indexcontains 16 chapters ... . The book is thus a valuable aid both for beginning and for experienced laboratory workers. ... Diagnostic Bacteriology Protocols will be used both by experienced molecular biologists for whom it is a source of extending their used techniques, and by beginning specialists with basic knowledge of diagnostic procedures in molecular biology. The uniform and clear treatment offers very good orientation in the flood of ever-emerging facts." (E. Pavlík, Folia Microbiologica, December, 2007)
"The text is lucid and wonderfully brief...The techniques described have universal application to bacteriological analyses regardless of one's special interests or focus...a well and necessary addendum for all laboratories engaged in or planning to perform bacterial identifications..." -Quarterly Review of Biology
"...a useful addition to the portfolio of bioscience laboratories" -Microbiology Today
"A very useful book for the bench scientist interested in techniques for differentiating bacterial isolates...should prove useful in a wide range of industrial and academic applications." -Doody's Health Sciences Book Review Journal
"...undoubtedly a useful addition to the series and would be of use to research scientists." -Journal of Clinical Pathology
From the reviews of the second edition:
"This concise volume contains simple introductions to many techniques to supplement the traditional agar plate ... . The text is easy and clear to follow ... . the aim of the book is to provide ideas. The chapters can, therefore, be read for inspiration ... . Step-by-step guide to many of the procedures are included, so those about to embark on a new technique, or even those simply wanting to understand the principles ... will be able to benefit. ... A handy reference ... ." (Dr. J Cargill, acp News, Winter, 2006)
"This very practical volume provides concise, state of the art molecular diagnostic bacteriology protocols for a range of species ... . In my view this book provides a valuable additional source of applied protocols for scientists interested in the detection and differentiation of bacteria ... . I strongly recommend this book for purchase by laboratory-based scientists interested in bacterial detection and differentiation." (David C. Coleman, SGM- Society for General Microbiology, 2007)
"The hard-cover publication in A5 format amounting to 225 pages including a six-page indexcontains 16 chapters ... . The book is thus a valuable aid both for beginning and for experienced laboratory workers. ... Diagnostic Bacteriology Protocols will be used both by experienced molecular biologists for whom it is a source of extending their used techniques, and by beginning specialists with basic knowledge of diagnostic procedures in molecular biology. The uniform and clear treatment offers very good orientation in the flood of ever-emerging facts." (E. Pavlík, Folia Microbiologica, December, 2007)