DNA Origami (eBook, PDF)

Structures, Technology, and Applications
Redaktion: Endo, Masayuki

• Format: PDF

Produktbeschreibung

DNA ORIGAMI Discover the impact and multidisciplinary applications of this subfield of DNA nanotechnology DNA origami refers to the technique of assembling single-stranded DNA template molecules into target two- and three-dimensional shapes at the nanoscale. This is accomplished by annealing templates with hundreds of DNA strands and then binding them through the specific base-pairing of complementary bases. The inherent properties of these DNA molecules--molecular recognition, self-assembly, programmability, and structural predictability--has given rise to intriguing applications from drug delivery systems to uses in circuitry in plasmonic devices. The first book to examine this important subfield, DNA Origami brings together leading experts from all fields to explain the current state and future directions of this cutting-edge avenue of study. The book begins by providing a detailed examination of structural design and assembly systems and their applications. As DNA origami technology is growing in popularity in the disciplines of chemistry, materials science, physics, biophysics, biology, and medicine, interdisciplinary studies are classified and discussed in detail. In particular, the book focuses on DNA origami used for creating new functional materials (combining chemistry and materials science; DNA origami for single-molecule analysis and measurements (as applied in physics and biophysics); and DNA origami for biological detection, diagnosis and therapeutics (medical and biological applications). DNA Origami readers will also find: * A complete guide for newcomers that brings together fundamental and developmental aspects of DNA origami technology * Contributions by a leading team of experts that bring expert views from different angles of the structural developments and applications of DNA origami * An emerging and impactful research topic that will be of interest in numerous multidisciplinary areas * A helpful list of references provided at the end of each chapter to give avenues for further study Given the wide scope found in this groundbreaking work, DNA Origami is a perfect resource for nanotechnologists, biologists, biophysicists, chemists, materials scientists, medical scientists, and pharmaceutical researchers.

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Produktdetails

• Verlag: Jossey-Bass
• Seitenzahl: 432
• Erscheinungstermin: 5. Mai 2022
• Englisch
• ISBN-13: 9781119682578
• Artikelnr.: 64064581

Autorenporträt

Masayuki Endo, PhD, is a project professor at Kansai University and a guest professor at Kyoto University, Japan. He received his PhD from the Department of Chemistry and Biotechnology, The University of Tokyo in 1997. Prof. Endo's research work involves DNA nanotechnology and single-molecule analysis.

Inhaltsangabe

List of Contributors xiii

Preface xvii

1 DNA Origami Technology: Achievements in the Initial 10 Years 1
Masayuki Endo

1.1 Introduction 1

1.1.1 DNA Nanotechnology Before the Emergence of DNA Origami 3

1.2 Two- Dimensional DNA Origami 3

1.3 Programmed Arrangement of Multiple DNA Origami Components 6

1.4 Three- Dimensional DNA Origami Structures 9

1.5 Modification and Functionalization of 2D DNA Origami Structures 11

1.5.1 Selective Placement of Functional Nanomaterials 11

1.5.2 Selective Placement of Functional Molecules and Proteins via Ligands 13

1.5.3 Distance- Controlled Enzyme Reactions and Photoreactions 13

1.6 Single- Molecule Detection and Sensing using DNA Origami Structures 14

1.6.1 Single- Molecule RNA Detection 14

1.6.2 Single- Molecule Detection of Chemical Reactions 14

1.6.3 Single- Molecule Detection using Mechanical DNA Origami 14

1.6.4 Single- Molecule Sensing using Mechanical DNA Origami 14

1.7 Application to Single Biomolecule AFM Imaging 16

1.7.1 High- Speed AFM- Based Observation of Biomolecules 16

1.7.2 Visualization of DNA Structural Changes in the DNA Nanospace 18

1.7.3 Visualization of the Reaction Events of Enzymes and Proteins in the DNA Nanospace 18

1.8 Single- Molecule Fluorescence Studies 19

1.8.1 Nanoscopic Ruler for Single- Molecule Imaging 19

1.8.2 Kinetics of Binding and Unbinding Events and DNA- PAINT 21

1.8.3 DNA Barcode Imaged by DNA- PAINT 21

1.9 DNA Molecular Machines 22

1.9.1 DNA Assembly Line Constructed on the DNA Origami 22

1.9.2 DNA Spider System Constructed on the DNA Origami 22

1.9.3 DNA Motor System Constructed on the DNA Origami 24

1.10 Selective Incorporation of Nanomaterials and the Applications 24

1.10.1 DNA Origami Plasmonic Structure with Chirality 24

1.10.2 Surface- Enhanced Fluorescence by Gold Nanoparticles and DNA Origami Structure 26

1.10.3 Placement of DNA Origami onto a Fabricated Solid Surface 26

1.11 Dynamic DNA Origami Structures Responsive to External Stimuli 27

1.11.1 DNA Origami Structures Responsive to External Stimuli 27

1.11.2 Stimuli- Responsive DNA Origami Plasmonic Structures 27

1.11.3 Photo- Controlled DNA Origami Plasmonic Structures 27

1.12 Conjugation of DNA Origami to Lipid 29

1.12.1 DNA Origami Channel with Gating 29

1.12.2 DNA Origami Templated Synthesis of Liposomes 29

1.13 DNA Origami for Biological Applications 29

1.13.1 Introduction of DNA Origami into Cells and Functional Expression 29

1.13.2 Drug Release Using the Properties Characteristic for DNA Origami 31

1.13.3 DNA Origami Structures Coated with Lipids and Polymers 32

1.13.4 Nanorobot with Dynamic Mechanism 32

1.13.5 Nanorobot Targeting Tumor In Vivo 32

1.14 Conclusions 33

References 34

2 Wireframe DNA Origami and Its Application as Tools for Molecular Force Generation 41
Marco Lolaico and Björn Högberg

2.1 Introduction 41

2.2 Pre- Origami Wireframe DNA Nanostructures 42

2.3 Hierarchical DNA Origami Wireframe 43

2.4 Entire DNA Origami Design 45

2.5 DNA Origami Wireframe as Tools for Molecular Force Application 50

2.5.1 Introduction 50

2.5.2 Results and Discussion 51

2.6 Conclusions 54

2.6.1 Materials and Methods 54

References 55

3 Capturing Structural Switching and Self- Assembly Events Using High- Speed Atomic Force Microscopy 59