DNA Topoisomerase Protocols (eBook, PDF)

Volume I: DNA Topology and Enzymes
Redaktion: Bjornsti, Mary-Ann; Osheroff, Neil

• Format: PDF

Produktbeschreibung

DNA Topoisomerase Protocols, I: DNA Topology and Enzymes brings together an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, and bacterial, yeast and mammalian expression systems. A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. DNA Topoisomerase Protocols, I: DNA Topology and Enzymes will be of immense value to the many basic scientists and clinicians who want better to understand and to exploit proficiently the wealth of recent discoveries about chromatin structure and its relation to gene expression, about DNA topoisomerases as the targets of antitumor and antibacterial agents, and about DNA repair.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Humana Press
• Seitenzahl: 327
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781592592593
• Artikelnr.: 64615866

Inhaltsangabe

to DNA Topoisomerases.- Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis.- Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers.- Analysis of Altered DNA Structures.- Purification of Supercoiled Plasmid DNA.- Purification and Use of DNA Minicircles with Different Linking Numbers.- Isolation of Kinetoplast DNA.- Isolation of Knotted DNA from Coliphage P4.- Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresis.- Sedimentation Analysis of Bacterial Nucleoid Structure.- Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy.- Methods for Analyzing DNA Bending.- Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombination.- Overexpression and Purification of Bacterial DNA Gyrase.- Overexpression and Purification of Bacterial DNA Topoisomerase I.- Overexpression and Purification of Escherichia coliDNA Topoisomerase III.- Overexpression and Purification of Bacterial Topoisomerase IV.- Purification of the Bacteriophage T4 Type II DNA Topoisomerase.- Overexpression and Purification of DNA Topoisomerase I from Yeast.- Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeast.- Purification of DNA Topoisomerase II from Drosophila melanogaster.- Purification of DNA Topoisomerase I from Human Placenta.- Purification of Baculovirus-Expressed Human DNA Topoisomerase I.- Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography.- Metabolic Labeling, Immunoprecipitation, and Two-Dimensional Tryptic Phosphopeptide Mapping of Human Topoisomerase II.- Immunoblot Analysis and Band Depletion Assays.- Visualization of DNA Topoisomerases by Electron Microscopy.- Appendix: Compendium of DNA Topoisomerase Sequences.

Rezensionen

"...an invaluable practical aid. The central and expanding role of these enzymes in basic cell biology together with their important pharmaceutical applications will ensure that book will remain relevant for some years to come."-Cell Biology International 2000

"The introductory chapter by the editors gives a beautifully brief and concise overview of the entire family of DNA topoisomerases and related enzymes and can be used as an Ariadne's thread to the key papers of the field. Another useful item is the appended compendium of topoisomerase sequences, which is likely to be helpful in designing expression vectors, deletion mutants, etc. Most of the other chapters contain a lot of useful information and easily followed procedures.a very valuable addition to the topoisomerase literature. It summarizes a lot of useful information for those engaged or getting engaged in research on DNA topology or topoisomerases. Those just wanting to carry out some of the analytical procedures will in most cases find what they need, i.e. a concise delineation of the analytical background, some detailed and easy to follow protocols, and a comprehensive guideline for the interpretation of the results. As such, the book definitely deserves a place on many laboratory shelves"-FEBS Letters

"The introductory chapter is one of the most concise and clearly presented summaries of our current knowledge that has been written, and it will be useful even for experts in the field....The volume concludes with a well-organized and useful compendium of toposomerase DNA sequences. Volume I is clearly a valuable source of methodological expertise for anyone studying topoisomerases."-New Biological Books

"...We recommend greatly this remarkable book to all our readers, without exception." - Cellular and Molecular Biology