Drosophila (eBook, PDF)

Methods and Protocols
Redaktion: Dahmann, Christian

• Format: PDF

Produktbeschreibung

With its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demonstrates the ongoing efforts to broaden the technical repertoire. In Drosophila: Methods and Protocols, leading experts contribute a collection of protocols covering standard techniques as well as novel methods. As a volume in the highly successful Methods in Molecular Biology(TM) series, the chapters contain brief introductions to the subject matter, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and essential, Drosophila: Methods and Protocols allows novices and experts alike to tap into the power of one hundred years of expertise involving this vital model system.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 438
• Erscheinungstermin: 19. Juli 2008
• Englisch
• ISBN-13: 9781597455831
• Artikelnr.: 64692012

Autorenporträt

Christian Dahmann, Max Planck Institut of Molecular Cell Biology and Genetics, Dresden, Germany

Inhaltsangabe

Drosophila melanogaster and the Development of Biology in the 20th Century.- Getting Started.- FlyBase.- The Use of P-Element Transposons to Generate Transgenic Flies.- The GAL4 System.- P-Element Mutagenesis.- EMS Screens.- RNAi Screening in Cultured Drosophila Cells.- Methods for Homologous Recombination in Drosophila.- Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis.- Cuticle Preparation of Drosophila Embryos and Larvae.- Immunolabeling of Embryos.- Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins.- Analysis of Neuromuscular Junctions.- Immunolabeling of Imaginal Discs.- Imaging Drosophila Pupal Wing Morphogenesis.- Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections.- Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues.- Linear RNA Amplification for the Production of Microarray Hybridization Probes.- An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs.- Extraction and Immunoblotting of Proteins From Embryos.- Purification of Drosophila Protein Complexes for Mass Spectrometry.- Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes.- Flow Cytometric Analysis of Drosophila Cells.- Drosophila Cell Lines as Model Systems and as an Experimental Tool.