Scientific Study from the year 2013 in the subject Biology - Micro- and Molecular Biology, Junagadh Agricultural University (Department of biotechnology), language: English, abstract: For a long time used many growth media like Potato Dextroxe agar, Capazedx agar, Rose Bengal agar for the isolation and identification of various type of fungi like Aspergillus, Penicillium, Tricoderma etc. but the preparation of above said medium procedure is very lengthy and the components are used in this medium is so coastally except Potato Dextroxe agar (PDA). the PDA medium widely used for the isolation of fungi because it is easy to prepare and cheap ingredients. But another medium Tomato agar we prepared is more easy to prepare than PDA and more Cheap than the PDA. The growth pattern of fungi on Tomato Agar compare to Growth pattern of fungi on PDA was not significantly difference so the tomato agar medium will be used for isolation of fungi alternate PDA which is cheap medium for the isolation of fungi and may widely used in research laboratories.