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Electron Crystallography of Soluble and Membrane Proteins (eBook, PDF)

Methods and Protocols

Redaktion: Schmidt-Krey, Ingeborg; Cheng, Yifan
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The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment. Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, inclu...

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