Epstein-Barr Virus Protocols (eBook, PDF)

Redaktion: Wilson, Joanna B.; May, Gerhard H. W.

• Format: PDF

Produktbeschreibung

The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 438
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781592592272
• Artikelnr.: 64701639

Inhaltsangabe

Genome and Transcript Analyses.- Epstein-Barr Virus.- Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR.- Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids.- Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs).- Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells.- Visualizing EBV Expression Patterns by FISH.- Viral Detection.- In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products.- Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections.- Detection of EBV Infection at the Single-Cell Level.- Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo.- Culture Methods.- Virus Isolation.- Generation of Lymphoblastoid Cell Lines (LCLs).- Cell Cycle Distribution of B-Lymphocytes and Cell Lines.- of Plasmid Vectors into Cells Via Electroporation.- Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene.- Transient Gene Expression and MACS Enrichment.- In Vitro Assays to Study Epithelial Cell Growth.- In Vitro Assays to Study Epithelial Cell Differentiation.- Cell Sensitivity Assays.- Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation.- Immune Assays.- Regression Assay.- Generation of Polyclonal EBV-Specific CTL Cultures and Clones.- Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs.- Limiting Dilution Assay.- Viral Protein Detection.- Antibodies for Detecting EBV Latent Proteins.- Detection of EBV Latent Proteins by Western Blotting.- Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis.- Protein-Protein, Protein-DNA, and Protein-RNA Interactions.- The Yeast Two-Hybrid Assay to Identify Interacting Proteins.- Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins.- Magnetic DNA Affinity Purification of a Cellular Transcription Factor.- Screening an Expression Library with a DNA Binding Site.- Analysis of DNA Binding Proteins by Mobility Shift Assay.- Analysis of RNA-Protein Interactions of the EBV-Encoded Small RNAs, the EBERs.- Protein Activity Assays.- Chimeric and Mutated Variants of LMP1.- Assaying the Activity of Kinases Regulated by LMP1.- In Vitro Assays for the Detection of Protein Tyrosine Phosphorylation and Protein Tyrosine Kinase Activities.- Tissue and In Vivo Protocols.- Analysis of Apoptosis in Tissue Sections.- Considerations in Generating Transgenic Mice.- In Vivo Assay of Cellular Proliferation.- Topical Chemical Carcinogen Treatment in Mice.- Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture.- Selection and Enrichment of B Cells from Lymphoid Tissues.- Detection of Immunoglobulin Gene Rearrangements.

Rezensionen

"Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer." - Anticancer Research "Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter." -Today's Life Science "This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive..." "Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology." -Microbiology Today "Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design." -Tumori "This book is highly recommended for collections serving researchers in the area of molecular biology." -E-Streams