Flow Cytometry and Cell Sorting (eBook, PDF)

Redaktion: Radbruch, Andreas

• Format: PDF

Produktbeschreibung

Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced. In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible. From the reviews of the 1st edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended and well worth purchasing." (Cytobios, GB) "Full practical details and useful hints are given. This book will prove to be very useful in the lab." (Comp Biochem Physiol A, GB) "..the book provides a good overview of many of the uses of flow cytometry and would certainly be a good starting point for any of the topics covered. It is easy to read and as such would provide a good introduction to newcomers to flow cytometry." (Austr J Med Science)

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Produktdetails

• Verlag: Springer Berlin Heidelberg
• Seitenzahl: 355
• Erscheinungstermin: 14. März 2013
• Englisch
• ISBN-13: 9783662041291
• Artikelnr.: 53086188

Inhaltsangabe

I Flow Cytometry.- 1 Operation of a Flow Cytometer.- II Immunofluorescence.- 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies.- 3 Immunofluorescence: Basic Considerations.- 4 Multicolor Immunofluorescence Analysis.- 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells.- 6 Cytometry and Sorting of Live Cells According to Their Secreted Products.- 7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes.- 8 Scatchard Analysis by Flow Cytometry.- 9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry.- III DNA and Proliferation.- 10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis.- 11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression.- 12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique.- IV Cellular Activation and Biochemistry.- 13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium.- 14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry.- 15 Biochemical Parameters of Cells Function.- 16 The FACS-Gal Assay.- 17 Ligand Acidification by Nonadherent Cells.- V Cell Sorting.- 18 Powerful Preselection.- 19 Magnetic Selection of Transfected Cells.- 20 High Gradient Magnetic Cells Sorting.- 21 Setup of a Flow Sorter.- 22 Sorting of Rare Cells.- 23 Fluorescence-Activated Chromosome Sorting.- 24 Analysis and Sorting of Plant Chromosomes by Flow Cytometry.- 25 Large Particle Sorting.- VI Safety.- 26 Biological and Laser Safety.- Appendices: Birgit Mechtold and Andreas Radbruch.- Suppliers.- Cytometry on the Internet.