Fluorescent Energy Transfer Nucleic Acid Probes (eBook, PDF)

Designs and Protocols
Redaktion: Didenko, Vladimir V.

• Format: PDF

Produktbeschreibung

Although fluorescent probes and assays, which use energy transfer (ET) for monitoring DNA reactions, have multiplied in recent years, until now there have been no manuals summarizing the many different protocols and probe designs. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is the first publication to provide such a collection. In the volume, hands-on experts-often the original developers of the technique-comprehensively describe a variety of fluorescent probes and devices that use ET, including molecular beacons, molecular break lights, TaqMan® probes, LUX(TM) primers, Invader® assay, aptazymes, DNAzymes, molecular machines, biosensors, and logic gates for molecular-scale computation. Merging work on nanotechnology and fluorescent probes, the authors assemble the first comprehensive treatment of all the key DNA- and RNA-based ET probes and present strategies for their optimized custom design. They discuss both fluorescence resonance energy transfer (FRET)-based and non-FRET-based constructs, and provide a complete set of techniques to monitor DNA and RNA reactions, such as hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions. Detailed protocols are provided for distance determination in protein-DNA complexes and the detection of topological DNA alterations, mutations, and single-nucleotide polymorphisms. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering an outline of the principles behind the technique, step-by-step detailed instructions, and comprehensive troubleshooting. Exhaustive and state-of-the-art, Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is an invaluable resource for both novice and experienced researchers who wish to use the latest developments in fluorescent probes in any field of molecular biology.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 372
• Erscheinungstermin: 4. Februar 2008
• Englisch
• ISBN-13: 9781597450690
• Artikelnr.: 64701798

Inhaltsangabe

Design of Energy Transfer Probes.- Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes.- Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers.- Energy Transfer Probes for DNA and RNA Hybridization Detection and Monitoring.- Detection of DNA Hybridization Using Induced Fluorescence Resonance Energy Transfer.- Detecting RNA/DNA Hybridization Using Double-Labeled Donor Probes With Enhanced Fluorescence Resonance Energy Transfer Signals.- Energy Transfer Probes for DNA Breaks Detection and DNA Cleavage Monitoring.- Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections.- Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids.- A Continuous Assay for DNA Cleavage Using Molecular Break Lights.- Monitoring of DNA Synthesis and Amplification Using Energy Transfer Probes.- Homogenous Detection of Nucleic Acids Using Self-Quenched Polymerase Chain Reaction Primers Labeled With a Single Fluorophore (LUX(TM) Primers).- Use of Self-Quenched, Fluorogenic LUX(TM) Primers for Gene Expression Profiling.- TaqMan® Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Quantification and Identification of bcr-abl Transcript Type.- Quantitative TaqMan® Assay for the Detection and Monitoring of Cytomegalovirus Infection in Organ Transplant Patients.- Real-Time Detection and Quantification of Telomerase Activity Utilizing Energy Transfer Primers.- DNA Sequence Analysis and Mutation Detection Using Fluorescence Energy Transfer.- Invader® Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation.- Real-Time Quantitative Polymerase Chain Reaction Analysis of MitochondrialDNA Point Mutation.- Multiplex Single-Nucleotide Polymorphism Detection by Combinatorial Fluorescence Energy Transfer Tags and Molecular Affinity.- High-Throughput Genotyping With Energy Transfer-Labeled Primers.- Determination of Distance and DNA Folding.- Distance Determination in Protein-DNA Complexes Using Fluorescence Resonance Energy Transfer.- Multi-Fluorophore Fluorescence Resonance Energy Transfer for Probing Nucleic Acids Structure and Folding.- Dna-Based Biosensors Utilizing Energy Transfer.- Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons.- Fluorescent Energy Transfer Readout of an Aptazyme-Based Biosensor.- Fluorescence Resonance Energy Transfer in the Studies of Guanine Quadruplexes.- Solution-Phase Molecular-Scale Computation With Deoxyribozyme-Based Logic Gates and Fluorescent Readouts.

Rezensionen

From the reviews:

"The content of the book provides a complete source of information in the DNA/RNA area and related fields. ... In brief, the book is a useful, practical source of information. ... The group of scientists and researchers ... that forms the potential market for this book can be extended with an important category: undergraduate and graduate students and postdoctoral researchers. Some materials can be adapted for interdisciplinary courses in molecular life sciences." (Dr. Ton Visser, Molecular Biotechnology, Vol. 34, September, 2006)

"Fluorescence Energy Transfer Nucleic Acid Probes, Design and Protocols is a timely book. It is the first concise collection of fluorescence methods and assays used in exploring biomolecular structure, dynamic and interaction. ... a rich compendium of information for the beginner in the field, as well as a source of ideas and inspiration for the advanced researcher. It is a book that should be on the shelf in all laboratories using fluorescent-probe techniques in biochemistry and molecular biology." (Sabine Müller, ChemBioChem, Vol. 8, 2007)