J. Thomas McClintock

Forensic DNA Analysis (eBook, ePUB)

A Laboratory Manual

• Format: ePub

Produktbeschreibung

This introductory text provides a contextual history and overview of the science and use of DNA typing methods. Logically organized, with clear, concise language, this manual ensures a fundamental understanding of forensic DNA analysis and a thorough background of the molecular techniques used to determine an individual's identity. The experiments cover DNA extraction, concentration, and assessment; DNA analysis using restriction fragment length polymorphisms; polymerase chain reaction and PCR-based typing tests; short tandem repeat analysis; and mitochondrial DNA analysis. There is also an extensive glossary of DNA typing terminology and basic terms used in molecular biology.

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Produktdetails

• Verlag: Taylor & Francis
• Erscheinungstermin: 19. Februar 2008
• Englisch
• ISBN-13: 9781040084229
• Artikelnr.: 70002087

Autorenporträt

J. Thomas McClintock

Inhaltsangabe

An Overview of Forensic DNA Analysis, Types of Biological Samples, Exercise 1
DNA Extraction, Exercise 2
Concentration of Extracted DNA, Exercise 3
Microcon Concentration and Purification of Extracted DNA, Exercise 4
Assessing the Quality and Quantity of Isolated DNA, Exercise 5
DNA Analysis Using Restriction Fragment Length, Polymorphism (RFLP), Polymerase Chain Reaction (PCR), Exercise 6
Polymerase Chain Reaction (PCR)
Based Tests: The AmpliType PM/DQA1 System, Exercise 7
Polymerase Chain Reaction (PCR)
Based Tests: The D1S80 System, Experiment 8
Polymerase Chain Reaction (PCR)
Based Tests: Short Tandem Repeat (STR) Analysis, Experiment 9
Polymerase Chain Reaction (PCR)
Based Tests: Short Tandem Repeat (STR) Analysis to Determine Paternity (a Case Study), Experiment 10
Polymerase Chain Reaction (PCR)
Based Tests: Y
Chromosome Short Tandem Repeat (Y
STR) Analysis (a Case Study), Exercise 11
Mitochondrial DNA (mtDNA) Analysis, Exercise 12
Assessment of Lumigraph and Autoradiograph Data, References, Appendices