In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.
Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.
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From the reviews of the second edition:
"The book is organized into four sections which draw on contributions from several experts in the field of oxidative stress. ... Based on a collection of articles in the four sections, the book is a good reference for those interested in free radical science including new methodologies to measure both oxidative stress and antioxidants using in vivo and in vitro models." (Silvia Berlanga de Moraes Barros, Brazilian Journal of Pharmaceutical Sciences, May, 2010)
"The book is organized into four sections which draw on contributions from several experts in the field of oxidative stress. ... Based on a collection of articles in the four sections, the book is a good reference for those interested in free radical science including new methodologies to measure both oxidative stress and antioxidants using in vivo and in vitro models." (Silvia Berlanga de Moraes Barros, Brazilian Journal of Pharmaceutical Sciences, May, 2010)