Functional Nucleic Acids for Analytical Applications (eBook, PDF)

• Format: PDF

Produktbeschreibung

Nature has long used nucleic acid aptamers and enzymes for regulatory activities, such as the recently discovered “riboswitches” involved in gene expression. The existence of a large array of natural and artificial functional nucleic acids has generated tremendous enthusiasm and new opportunities for molecular scientists from diverse disciplines to devise new concepts and real applications that take advantage of those nucleic acids for sensing and other analytical applications. This book provides a timely and comprehensive overview of recent advances in the field, from leading experts in biology, chemistry, and engineering. A variety of topics are covered, from fundamentals of functional nucleic acids, to their applications as sensors, to nanotechnologies; as well as integration of functional nucleic acids into practical analytical systems.

Produktdetails

• Verlag: Springer New York
• Erscheinungstermin: 27. Mai 2009
• Englisch
• ISBN-13: 9780387737119
• Artikelnr.: 37287423

Inhaltsangabe

Overview of Functional Nucleic Acids and Their Analytical Applications.- Introductory Remarks.- Natural Functional Nucleic Acids: Ribozymes and Riboswitches.- Artificial Functional Nucleic Acids: Aptamers, Ribozymes, and Deoxyribozymes Identified by In Vitro Selection.- Functional Nucleic Acid Sensors Based on Different Transduction Principles.- Fluorescent Aptamer Sensors.- Fluorescent Ribozyme and Deoxyribozyme Sensors.- Colorimetric and Fluorescent Biosensors Based on Directed Assembly of Nanomaterials with Functional DNA.- Electrochemical Approaches to Aptamer-Based Sensing.- Amplified DNA Biosensors.- Other Emerging Analytical Applications.- Aptamers in Affinity Separations:Capillary Electrophoresis.- Aptamers in Affinity Separations: Stationary Separation.- Aptamer Microarrays.- The Use of Functional Nucleic Acids in Solid-Phase Fluorimetric Assays.- Functional Nucleic Acid Sensors as Screening Tools.- Nucleic Acids for Computation.- DNAzymes in DNA Nanomachines and DNA Analysis.

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Overview of Functional Nucleic Acids and Their Analytical Applications.- Introductory Remarks.- Natural Functional Nucleic Acids: Ribozymes and Riboswitches.- Artificial Functional Nucleic Acids: Aptamers, Ribozymes, and Deoxyribozymes Identified by In Vitro Selection.- Functional Nucleic Acid Sensors Based on Different Transduction Principles.- Fluorescent Aptamer Sensors.- Fluorescent Ribozyme and Deoxyribozyme Sensors.- Colorimetric and Fluorescent Biosensors Based on Directed Assembly of Nanomaterials with Functional DNA.- Electrochemical Approaches to Aptamer-Based Sensing.- Amplified DNA Biosensors.- Other Emerging Analytical Applications.- Aptamers in Affinity Separations:Capillary Electrophoresis.- Aptamers in Affinity Separations: Stationary Separation.- Aptamer Microarrays.- The Use of Functional Nucleic Acids in Solid-Phase Fluorimetric Assays.- Functional Nucleic Acid Sensors as Screening Tools.- Nucleic Acids for Computation.- DNAzymes in DNA Nanomachines and DNA Analysis.

Rezensionen

From the reviews: "This book consists of 15 chapters contributed by experts in the field of functional nucleic acids, mostly in the area of specific analytical applications of nucleic acids ... . this book affords a comprehensive collection of clearly presented, up-to-date reviews that broadly cover the numerous applications of functional nucleic acids. ... The chapters are of high quality ... that will be useful to newcomers entering the field of functional nucleic acids and to those interested in exploring other applications complementary to their own niches." (Jennifer S. Brodbelt, Journal of the American Chemical Society, Vol. 131 (32), 2009)