Gap Junction Protocols (eBook, PDF)

Redaktion: Vinken, Mathieu; Johnstone, Scott R.

• Format: PDF

Produktbeschreibung

Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.

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Produktdetails

• Verlag: Springer New York
• Seitenzahl: 235
• Erscheinungstermin: 20. Mai 2016
• Englisch
• ISBN-13: 9781493936649
• Artikelnr.: 64615512

Inhaltsangabe

Analysis of Liver Connexin ExpressionUsing Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.- DNA Methylation Analysis of HumanTissue-Specific Connexin Genes.- Detection of Connexins in Liver Cells UsingSodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.- Immunohisto- andCytochemistry Analysis of Connexins.- Small Interfering RNA-MediatedConnexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.- Generation and Use of TrophoblastStem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancyand Labor.- Identification of Connexin43Phosphorylation and S-Nitrosylationin Cultured Primary Vascular Cells.- Preparation of Gap Junctions inMembrane Microdomains for Immunoprecipitation and Mass Spectrometry InteractomeAnalysis.- Scrape Loading/Dye Transfer Assay.- Microinjection Technique for Assessment of GapJunction Function.- Electroporation Loading and DyeTransfer: A Safe and Robust Method to Probe Gap Junctional Coupling.- Using Fluorescence Recovery After Photobleaching to Study Gap JunctionalCommunication In Vitro.- Tracking Dynamic Gap Junctional Coupling in Live Cells by LocalPhoto-Activation and Fluorescence Imaging.- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication ofSecond Messengers in Cultured Bone Cells.- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Methodfor Studying Gap Junctions and Hemichannels.- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration forthe Measurement of Gap Junction Conductance.