William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman

Gene Biotechnology (eBook, PDF)

• Format: PDF

Produktbeschreibung

The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. The book also discusses approaches to grant funding and SiRNA technology. This edition features new techniques in every chapter, as well as several new chapters.

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Produktdetails

• Verlag: Taylor & Francis
• Seitenzahl: 576
• Erscheinungstermin: 19. April 2016
• Englisch
• ISBN-13: 9781439848326
• Artikelnr.: 38300071

Autorenporträt

William Wu, Ph.D., has extensive experience in both academic and industrial settings. He held various positions, including Research Assistant Professor at the University of Michigan Medical School, Ann Arbor, Michigan, USA, and Professor at the Hunan Nonnal University, Hunan, the People's Republic of China, Senior Scientist, Team-Leader and Chief Technology Officer (CTO) in biomedical and pharmaceutical companies.

Helen H. Zhang, MS., is Senior Research Associate at the University of Michigan Medical School, Ann Arbor, Michigan, USA. In 1996, she received her MS degree in Molecular and Cellular Biology at the Eastern Michigan University, Ypsilanti, Michigan, USA.

Michael J. Welsh, Ph.D., is Professor of Cell Biology in the Department of Cell and Developmental Biology at the University of Michigan Medical School, Ann Arbor, Michigan, USA. He is also a Professor of Toxicology in the School of Public Health at the University of Michigan, Ann Arbor.

Peter B. Kanfman, Ph.D., is Professor of Biology in the Plant Cellular and Molecular Biology Program in the Department of Biology and a member of the faculty of the Bioengineering Program at the University of Michigan.

Inhaltsangabe

Strategies for Novel Research Projects and Research Grant Funding. Rapid
Isolation of Specific cDNA or Gene by PCR. Construction and Screening of
Subtracted and Complete Expression cDNA Libraries. Subcloning of Genes or
DNA Fragments. Non-isotopic and Isotopic DNA or RNA Sequencing. Information
Superhighway and Computer Databases of Nucleic Acids & Proteins.
Characterization of DNA or Genes by Southern Blot Hybridization. Gene
Over-expression by Sense RNA in Mammalian System. Gene Under-expression by
Antisense DNA and RNA Strategies. Analysis of Gene Expression at the
Functional Genomic Level Using Northern blotting and/or PCR. Analysis of
Gene Expression at the Proteomic Level via Western Blotting. Analysis of
Protein Expression Using Immuno-fluorescence Localization. Analysis of
Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization
(RISH). Localization of DNA or Abundance mRNA by Fluorescence In Situ
Hybridization (FISH). In Situ PCR Hybridization of Low Copy Gene and In
Situ RT-PCR. Detection of Low Abundance mRNA. Isolation and
Characterization of Genes from Genomic DNA Libraries. Mouse Stem Cells as a
Model Mammalian Cell line for Gene Expression. New Strategies for Gene
Knockout. Large-scale Expression and Purification of Recombinant Proteins.
Quantitative Analysis of Functional Genome by Real-Time RT -PCR.
High-throughput Analysis of Gene Expression by DNA Microarrays (Gene
Chips). Construction and Screening of Human Antibody Libraries Using Phage
Display Technology. Down-regulation of Gene Expression in Mammalian Systems
via SiRNA Technology. New Techniques for Identification of Protein Binding
and Proteomic Interactions.