T. A. Brown

Gene Cloning and DNA Analysis (eBook, PDF)

An Introduction

• Format: PDF

Produktbeschreibung

Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within.

Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like:

• The strategies used by researchers and industry practitioners to assemble genome sequences
• Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes
• Includes the use and application of gene editing strategies
• Interbreeding between Neanderthals and Homo Sapiens

Gene Cloning and DNA Analysis: An Introduction, 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.

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Produktdetails

• Verlag: John Wiley & Sons
• Erscheinungstermin: 30. September 2020
• Englisch
• ISBN-13: 9781119640752
• Artikelnr.: 60406359

Autorenporträt

T. A. Brown is Emeritus Professor of Biomolecular Archaeology in the Manchester Institute of Biotechnology at the University of Manchester in the United Kingdom. He has published several books on genetics, genomics and biochemistry as well as over 150 research papers.

Inhaltsangabe

Preface to the Eighth Edition xv

Part I The Basic Principles of Gene Cloning and DNA Analysis 1

1 Why Gene Cloning and DNA Analysis are Important 3

2 Vectors for Gene Cloning: Plasmids and Bacteriophages 15

3 Purification of DNA from Living Cells 29

4 Manipulation of Purified DNA 53

5 Introduction of DNA into Living Cells 83

6 Cloning Vectors for E. coli 101

7 Cloning Vectors for Eukaryotes 121

8 How to Obtain a Clone of a Specific Gene 145

9 The Polymerase Chain Reaction 169

Part II The Applications of Gene Cloning and DNA Analysis in Research 187

10 Sequencing Genes and Genomes 189

11 Studying Gene Expression and Function 217

12 Studying Genomes 243

13 Studying Transcriptomes and Proteomes 259

Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 275

14 Production of Protein from Cloned Genes 277

15 Gene Cloning and DNA Analysis in Medicine 301

16 Gene Cloning and DNA Analysis in Agriculture 327

17 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 355

Glossary 377

Index 395

Preface to the Eighth Edition xv

Part I The Basic Principles of Gene Cloning and DNA Analysis 1

1 Why Gene Cloning and DNA Analysis are Important 3

1.1 The early development of genetics 4

1.2 The advent of gene cloning and the polymerase chain reaction 4

1.3 What is gene cloning? 5

1.4 What is PCR? 5

1.5 Why gene cloning and PCR are so important 8

1.5.1 Obtaining a pure sample of a gene by cloning 8

1.5.2 PCR can also be used to purify a gene 10

1.6 How to find your way through this book 11

Further reading 13

2 Vectors for Gene Cloning: Plasmids and Bacteriophages 15

2.1 Plasmids 15

2.1.1 Size and copy number 17

2.1.2 Conjugation and compatibility 18

2.1.3 Plasmid classification 19

2.1.4 Plasmids in organisms other than bacteria 19

2.2 Bacteriophages 19

2.2.1 The phage infection cycle 20

2.2.2 Lysogenic phages 20

2.2.3 Viruses as cloning vectors for other organisms 26

Further reading 27

3 Purification of DNA from Living Cells 29

3.1 Preparation of total cell DNA 30

3.1.1 Growing and harvesting a bacterial culture 30

3.1.2 Preparation of a cell extract 31

3.1.3 Purification of DNA from a cell extract 33

3.1.4 Concentration of DNA samples 37

3.1.5 Measurement of DNA concentration 38

3.1.6 Other methods for the preparation of total cell DNA 39

3.2 Preparation of plasmid DNA 40

3.2.1 Separation on the basis of size 41

3.2.2 Separation on the basis of conformation 42

3.2.3 Plasmid amplification 44

3.3 Preparation of bacteriophage DNA 46

3.3.1 Growth of cultures to obtain a high lambda titre 47

3.3.2 Preparation of non-lysogenic lambda phages 47

3.3.3 Collection of phages from an infected culture 49

3.3.4 Purification of DNA from lambda phage particles 49

3.3.5 Purification of M13 DNA causes few problems 49

Further reading 51

4 Manipulation of Purified DNA 53

4.1 The range of DNA manipulative enzymes 55

4.1.1 Nucleases 55

4.1.2 Ligases 57

4.1.3 Polymerases 57

4.1.4 DNA modifying enzymes 58

4.2 Enzymes for cutting DNA - restriction endonucleases 59

4.2.1 The discove