Sie sind bereits eingeloggt. Klicken Sie auf 2. tolino select Abo, um fortzufahren.
Bitte loggen Sie sich zunächst in Ihr Kundenkonto ein oder registrieren Sie sich bei bücher.de, um das eBook-Abo tolino select nutzen zu können.
A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.
A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.
Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.
Die Herstellerinformationen sind derzeit nicht verfügbar.
Inhaltsangabe
Part 1 Overview: general comparison of vectors and DNA transfer procedures; transient and stable expression systems; viral compared to DNA mediated transfer; stratefic overview - matching experimental procedures with goals and probability of success. Part 2 Vectors (DNA): vector components - organization of the most efficient vectors such as pMT2, pCD, SRa, EBV based, BPV based and T7 based vectors; control elements; retroviral vectors and recombinant retroviruses; PCR based expression; homologous recombination and gene replacement. Part 3 Cell lines and maintenance: general tissue culture techniques; growth conditions of most commonly used mammalian cells - COS, 3t3, CV1, CHO, C127, BHK, mouse L cells, Att20, MDCK, lymphoid cells; embryonal stem cells. Part 4 DNA transfer: calcium phosphate; DEAE dextran; protoplast fusion; electroporation; chromosome mediated transfer - DEPC/PEF fusion, gamma irradiation/PEG fusion; selecting transfer technique for cell type and stability of expression. Part 5 Selection and amplification methods: DHER; Ada; Neo; GPT; Hygro; ODC, Asn synthtase, mdr; negative selection protocols (gancyclovir). Part 6 Expression cloning: library construction; transfaction techniques; SIB selection. Part 7 Analysis of nucleic acid: isolation of RNA and DNA; analysis of RNA (traditional and PCR based); analysis of DNA (traditional and PCR based). Part 8 Analysis of protein: radiolabeling, immunoprecipitation and PAGE systems; western immunoblotting; analysis of glycosylation, sulfation and acylation; study of protein processing and secretion. Appendix: reagents - where to obtain and how to prepare; troubleshooting.
Part 1 Overview: general comparison of vectors and DNA transfer procedures; transient and stable expression systems; viral compared to DNA mediated transfer; stratefic overview - matching experimental procedures with goals and probability of success. Part 2 Vectors (DNA): vector components - organization of the most efficient vectors such as pMT2, pCD, SRa, EBV based, BPV based and T7 based vectors; control elements; retroviral vectors and recombinant retroviruses; PCR based expression; homologous recombination and gene replacement. Part 3 Cell lines and maintenance: general tissue culture techniques; growth conditions of most commonly used mammalian cells - COS, 3t3, CV1, CHO, C127, BHK, mouse L cells, Att20, MDCK, lymphoid cells; embryonal stem cells. Part 4 DNA transfer: calcium phosphate; DEAE dextran; protoplast fusion; electroporation; chromosome mediated transfer - DEPC/PEF fusion, gamma irradiation/PEG fusion; selecting transfer technique for cell type and stability of expression. Part 5 Selection and amplification methods: DHER; Ada; Neo; GPT; Hygro; ODC, Asn synthtase, mdr; negative selection protocols (gancyclovir). Part 6 Expression cloning: library construction; transfaction techniques; SIB selection. Part 7 Analysis of nucleic acid: isolation of RNA and DNA; analysis of RNA (traditional and PCR based); analysis of DNA (traditional and PCR based). Part 8 Analysis of protein: radiolabeling, immunoprecipitation and PAGE systems; western immunoblotting; analysis of glycosylation, sulfation and acylation; study of protein processing and secretion. Appendix: reagents - where to obtain and how to prepare; troubleshooting.
Es gelten unsere Allgemeinen Geschäftsbedingungen: www.buecher.de/agb
Impressum
www.buecher.de ist ein Internetauftritt der buecher.de internetstores GmbH
Geschäftsführung: Monica Sawhney | Roland Kölbl | Günter Hilger
Sitz der Gesellschaft: Batheyer Straße 115 - 117, 58099 Hagen
Postanschrift: Bürgermeister-Wegele-Str. 12, 86167 Augsburg
Amtsgericht Hagen HRB 13257
Steuernummer: 321/5800/1497
USt-IdNr: DE450055826
