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While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly. Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and…mehr
While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly. Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and analysis, DNA microarrays, 2D electrophoresis and 2D chromatography, mass spectrometry and high-throughput protein expression and purification. The book also features examples of the exploitation of genomics and post-genomics in vaccine discovery, and contains useful descriptions of the biology and pathogenesis of clinically important bacterial pathogens. This book should be of interest to all those working in vaccine discovery and development in pharmaceutical and biotechnology companies as well as in academic institutions
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Guido Grandi is the editor of Genomics, Proteomics and Vaccines, published by Wiley.
Inhaltsangabe
Preface. List of Contributors. PART 1: INTRODUCTION. 1. Vaccination: Past, Present and Future (Maria Lattanzi and Rino Rappuoli). 2. Bioinformatics, DNA Microarrays and Proteomics in Vaccine Discovery: Competing or Complementary Technologies? (Guido Grandi). PART 2: TECHNOLOGIES. 3. Genome Sequencing and Analysis (Hervé Tettelin and Tamara Feldblyum). 4. Understanding DNA Microarrays: Sources and Magnitudes of Variances in DNA Microarray Data Sets (She-pin Hung, G. Wesley Hatfield, Suman Sundaresh and Pierre F. Baldi). 5. The proteome, Anno Domini Two Zero Zero Three (Pier Giorgio Righetti, Mahmoud Hamdan, Frederic Reymond and Joël S. Rossier). 6. Mass Spectrometry in Proteomics (Pierre-Alain Binz). 7. High Throughput Cloning, Expression and Purification Technologies (Andreas Kreusch and Scott A. Lesley). PART 3: APPLICATIONS. 8. Meningococcus B: from Genome to Vaccine (Davide Serruto, Rino Rappuoli and Mariagrazia Pizza). 9. Vaccines Against Pathogenic Streptococci (John L. Telford, Immaculada Margarit y Ros, Domenico Maione, Vega Masignani, Hervé Tettelin, Giuliano Bensi and Guido Grandi). 10. Identification of the 'Antigenome'-a Novel Tools for Design and Development of Subunit Vaccines Against Bacterial Pathogens (Eszter Nagy, Tamás Henics, Alexander von Gabain and Andreas Meinke). 11. Searching the Chlamydia Genomes for New Vaccine Candidates (Giulio Ratti, Oretta Finco and Guido Grandi). 12. Proteomics and Anti-Chlamydia Vaccine Discovery (Gunna Christiansen, Svend Birkelund, Brian B. Vandahl and Allan C Shaw). 13. Proteome Analysis of Other Membrane and Extracelular Proteins from Pseudomonas aeruginosa for vaccine Discovery (Stuart J. Cordwell and Amanda S. Nouwens). Index.
Preface. List of Contributors. PART 1: INTRODUCTION. 1. Vaccination: Past, Present and Future (Maria Lattanzi and Rino Rappuoli). 2. Bioinformatics, DNA Microarrays and Proteomics in Vaccine Discovery: Competing or Complementary Technologies? (Guido Grandi). PART 2: TECHNOLOGIES. 3. Genome Sequencing and Analysis (Hervé Tettelin and Tamara Feldblyum). 4. Understanding DNA Microarrays: Sources and Magnitudes of Variances in DNA Microarray Data Sets (She-pin Hung, G. Wesley Hatfield, Suman Sundaresh and Pierre F. Baldi). 5. The proteome, Anno Domini Two Zero Zero Three (Pier Giorgio Righetti, Mahmoud Hamdan, Frederic Reymond and Joël S. Rossier). 6. Mass Spectrometry in Proteomics (Pierre-Alain Binz). 7. High Throughput Cloning, Expression and Purification Technologies (Andreas Kreusch and Scott A. Lesley). PART 3: APPLICATIONS. 8. Meningococcus B: from Genome to Vaccine (Davide Serruto, Rino Rappuoli and Mariagrazia Pizza). 9. Vaccines Against Pathogenic Streptococci (John L. Telford, Immaculada Margarit y Ros, Domenico Maione, Vega Masignani, Hervé Tettelin, Giuliano Bensi and Guido Grandi). 10. Identification of the 'Antigenome'-a Novel Tools for Design and Development of Subunit Vaccines Against Bacterial Pathogens (Eszter Nagy, Tamás Henics, Alexander von Gabain and Andreas Meinke). 11. Searching the Chlamydia Genomes for New Vaccine Candidates (Giulio Ratti, Oretta Finco and Guido Grandi). 12. Proteomics and Anti-Chlamydia Vaccine Discovery (Gunna Christiansen, Svend Birkelund, Brian B. Vandahl and Allan C Shaw). 13. Proteome Analysis of Other Membrane and Extracelular Proteins from Pseudomonas aeruginosa for vaccine Discovery (Stuart J. Cordwell and Amanda S. Nouwens). Index.
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