Germline Stem Cells (eBook, PDF)

Redaktion: Hou, Steven X.; Singh, Shree Ram

• Format: PDF

Produktbeschreibung

In this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. The volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 273
• Erscheinungstermin: 14. März 2008
• Englisch
• ISBN-13: 9781603272148
• Artikelnr.: 64701608

Inhaltsangabe

Identification and Regulation of Germline Stem Cells in Model Organisms.- The Development of Germline Stem Cells in Drosophila.- Analysis of the C. elegans Germline Stem Cell Region.- Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell.- Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells.- Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus).- High-Resolution Light Microscopic Characterization of Spermatogonia.- Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes.- Epigenetic Control in Male Germ Cells.- GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro.- In Vitro Culture and Applications of Germline Stem Cells.- Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts.- Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice.- Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture.- Deriving Mouse Spermatogonial Stem Cell Lines.- Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes.- Testicular Germ Cell Tumors in Mice.- Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries.

Rezensionen

From the reviews: "This book provides background information and protocols for identifying, isolating, characterizing, and manipulating ... germline stem cells. ... This book is written for basic science and clinical researchers ... as well as those interested in development and reproductive biology. Biomedical scientists and clinicians ... will also appreciate the wealth of new information. ... This is an outstanding contribution to the literature in germ cell biology. ... will be valued by all those interested in development of the germ lineage and stem cell biology." (Bruce A. Fenderson, Doody's Review Service, October, 2008)