Now in its second edition, this updated text examines new advances and concepts in the field. Topics include monolithic columns, bonded stationary phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, retention models for ions, and capillary electromigration techniques. It addresses HPLC detectors, LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, polymer HPLC, field-flow fractionation, affinity and ion chromatography. With figures, tables, and references, this volume explores significant areas of HPLC application including chiral pharmaceutical, environmental, food, and forensic analysis.
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