Heterologous Expression of Membrane Proteins (eBook, PDF)

Methods and Protocols
Redaktion: Mus-Veteau, Isabelle

• Format: PDF

Produktbeschreibung

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 322
• Erscheinungstermin: 2. August 2016
• Englisch
• ISBN-13: 9781493936373
• Artikelnr.: 64701334

Inhaltsangabe

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functionaland Structural Studies.- Membrane Protein Production in the Yeast, S. cerevisiae.- Membrane ProteinsProduction in Escherichia coli:Protocols and Rules.- Codon Optimizing for Increased Membrane ProteinProduction: A Minimalist Approach.- Generation of Tetracycline-InducibleMammalian Cell Lines by Flow Cytometry for Improved Overproduction of MembraneProteins.- Membrane Protein Production in Lactococcus lactis for FunctionalStudies.- Expression ofViral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.- Leishmania tarentolæ as a Promising Toolfor Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic MembraneProteins: The Case of the ABC Pump ABCG6.- Overexpression, MembranePreparation, and Purification of a Typical Multidrug ABC Transporter BmrA.- Expression ofEukaryotic Membrane Proteins in Pichiapastoris.- Integral Membrane Protein Expression in Saccharomyces cerevisiae.- High-Throughput Baculovirus ExpressionSystem for Membrane Protein Production.- Small-Scale Screening to Large-ScaleOver-Expression of Human Membrane Proteins for Structural Studies.- Purificationof Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies.- MembraneProtein Solubilization and Composition of Protein Detergent Complexes.- Detergent-FreeMembrane Protein Purification.- Conformational Dynamics and Interactions ofMembrane Proteins by Hydrogen/Deuterium Mass Spectrometry.- Lessons from an a-Helical Membrane Enzyme: Expression,Purification, and Detergent Optimization for Biophysical and StructuralCharacterization.- Method to Screen Multidrug Transport Inhibitors Using YeastOver-Expressing a Human MDR Transporter.