High Throughput Screening Methods (eBook, PDF)

Evolution and Refinement
Redaktion: Bittker, Joshua A; Ross, Nathan T

• Format: PDF

Produktbeschreibung

High throughput screening remains a key part of early stage drug and tool compound discovery, and methods and technologies have seen many fundamental improvements and innovations over the past 20 years. This comprehensive book provides a historical survey of the field up to the current state-of-the-art. In addition to the specific methods, this book also considers cultural and organizational questions that represent opportunities for future success.
Following thought-provoking foreword and introduction from Professor Stuart Schreiber and the editors, chapters from leading experts across academia and industry cover initial considerations for screening, methods appropriate for different goals in small molecule discovery, newer technologies that provide alternative approaches to traditional miniaturization procedures, and practical aspects such as cost and resourcing. Within the context of their historical development, authors explain common pitfalls and their solutions.
This book will serve as both a practical reference and a thoughtful guide to the philosophy underlying technological change in such a fast-moving area for postgraduates and researchers in academia and industry, particularly in the areas of chemical biology, pharmacology, structural biology and assay development.

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Produktdetails

• Verlag: RSC
• Seitenzahl: 396
• Erscheinungstermin: 5. Dezember 2016
• Englisch
• ISBN-13: 9781782626770
• Artikelnr.: 47390646

Inhaltsangabe

Perspective/overview: State of the art in HTS at the academic-industry interface
Assay design and optimization
Assessment of available compound collections
Novel approaches to discovery and analysis of interesting compound combinations
Modern biophysical methods in target-based screening
Genetic perturbation methods
Understanding luminescence-based screens
Measuring interactions in live cells
Approaches to high-content imaging and multi-feature analysis
Profiling of genetically characterized cell systems
Multidimensional profile-based screening
Using biological systems that better recapitulate physiology
Phenotypic screens with model organisms
Encoded libraries
Modern virtual screening
Research data management
Small molecule bioactivity databases
Chemical biology in a pharma environment
Practical aspects of funding and executing high-throughput screens