R. L. M Pierik

In Vitro Culture of Higher Plants (eBook, PDF)

• Format: PDF

Produktbeschreibung

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

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Produktdetails

• Verlag: Springer Netherlands
• Seitenzahl: 348
• Erscheinungstermin: 6. Dezember 2012
• Englisch
• ISBN-13: 9789401157506
• Artikelnr.: 44182209

Inhaltsangabe

2. Introduction.- 2.1. Outline.- 2.2. Abbreviations and glossary.- 3. History.- 3.1. Introduction.- 3.2. Developments in the Netherlands and other countries.- 4. Types of cultures.- 5. Laboratory equipment.- 5.1. Supplies.- 5.2. The laminar air-flow cabinet.- 5.3. Sterilization of nutrient media.- 5.4. Preparation room.- 5.5. Cleaning glassware.- 5.6. Robots in the plant tissue culture laboratory.- 6. Preparation and composition of nutrient media.- 6.1. Introduction.- 6.2. Glassware and plastics.- 6.3. Preparation.- 6.4. Composition.- 6.5. Commercially prepared media.- 6.6. Storage of nutrient media.- 7. Closure of test tubes and flasks.- 8. Care of plant material.- 9. Sterilization of plant material.- 9.1. Introduction.- 9.2. Chemical sterilization.- 9.3. Apparently sterile cultures.- 9.4. Internal infections.- 9.5. Symbiotic cultures.- 10. Isolation, inoculation and subculturing.- 10.1. Introduction.- 10.2. Isolation.- 10.3. Inoculation.- 10.4. Subculturing.- 11. Mechanization.- 12. The influence of plant material on growth and development.- 13. The influence of physical factors on growth and development.- 13.1. The culture room.- 13.2. Discussion of special physical factors.- 14. The transfer from nutrient medium to soil.- 15. Aids to study.- 15.1. Literature study.- 15.2. Societies and associations.- 15.3. Laboratory notebook, photographs and slides.- 16. Embryo culture.- 16.1. Introduction.- 16.2. Techniques.- 16.3. Factors affecting the success of embryo culture.- 16.4. Practical applications.- 17. Germination of orchid seeds.- 17.1. Introduction.- 17.2. Factors affecting germination and growth.- 18. Vegetative propagation of orchids.- 18.1. Introduction.- 18.2. Meristem culture.- 18.3. Other methods of propagation.- 18.4. Variation arising during culture.- 18.5.Practical applications.- 19. Production of disease-free plants.- 19.1. Introduction.- 19.2. Production of virus-free plants.- 19.3. Production of bacteria- and fungal-free plants by meristem culture.- 20. Vegetative propagation.- 20.1. General introduction.- 20.2. Single-node culture.- 20.3. The axillary bud method.- 20.4. Regeneration of explants.- 20.5. Callus induction, callus culture and regeneration of organs and embryos.- 20.6. Regeneration of plants from single cells.- 20.7. Synthetic seeds.- 21. Somaclonal variation.- 22. Test tube fertilization.- 23. Production of haploids.- 23.1. General introduction.- 23.2. Obtaining haploids in vitro.- 23.3. Problems associated with haploid induction.- 24. Genetic manipulation.- 24.1. General introduction.- 24.2. Description.- 24.3. Prerequisites for the use of genetic manipulation.- 24.4. Somatic hybridization.- 24.5. The relevance of somatic hybridization.- 24.6. Disadvantages and problems of somatic hybridization.- 24.7. Selection procedures after somatic hybridization.- 24.8. Selection of mutants.- 24.9. Transformation by Agrobacterium tumefaciens.- 25. Miscellaneous applications.- 25.1. In phytopathology.- 25.2. In plant breeding.- 25.3. The biosynthesis of substances in vitro.- 26. In vitro cloning of plants in the Netherlands.- 27. Index.- 28. Literature cited.