In Vitro Fertilization (eBook, PDF)

A Textbook of Current and Emerging Methods and Devices
Redaktion: Nagy, Zsolt Peter; Agarwal, Ashok; Varghese, Alex C.

• Format: PDF

Produktbeschreibung

Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world.

With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.

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Produktdetails

• Verlag: Springer Nature Switzerland
• Seitenzahl: 962
• Erscheinungstermin: 3. Juli 2019
• Englisch
• ISBN-13: 9783319430119
• Artikelnr.: 57626824

Autorenporträt

Zsolt Peter Nagy, MD, PhD Scientific Director Reproductive Biology Associates - Prelude Atlanta, GA, USA Alex C Varghese, PhD Scientific Director Astra Fertility Group Mississauga, ON, Canada Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), American Center for Reproductive Medicine Cleveland Clinic Cleveland, OH, USA

Inhaltsangabe

Journey of Human Gametes In Vitro
1978 to 2018.
 Setting up an ART unit
Planning, Design and Construction.
 Building the Laboratory.
 Air Quality Management.
 Organization And Workflow In A Fertility Clinic.
 Risk and Safety in the IVF Clinic.
 Control of variables.
 Daily, Weekly, and Regular Preparations for the IVF Laboratory.
 Essential Instruments and Disposable Supplies for an IVF Laboratory.
 Co2 and Low
O2 Incubators.
 What to consider when selecting a LAF, Class II Cabinet or Isolette for your ART program?.
 Culture Media in IVF: Decisions for the Laboratory.
 Assessment Of Oocyte Quality.
 Oocyte Denuding.
 Interrupted or Uninterrupted Culture to the Blastocyst Stage in a Single Medium.
 Sequential Media for Human Blastocyst Culture.
 In Vitro Maturation of Human Oocytes.
 Design and Development of Simplified, Low Cost Technologies for Clinical IVF: Applications in High and Low Resource Settings.
 In vitro culture of oocytes and embryos with micro
vibration: "naturalization" of routine IVF procedure.
 Microfluidics for Gamete Manipulation and Embryo Culture.
 Time
Lapse Microscopy for Embryo Culture and Selection.
 Sperm Assessment: Traditional Approaches and Their Indicative Value.
 Sperm Assessment: Novel Approaches and Their Indicative Value.
 Assessment of Sperm Chromatin Damage by TUNEL Method Using Bench Top Flow Cytometer.
 Sperm processing in ART.
 PESA/MESA/TESA/TESE Sperm Processing.
 Processing Sperm Samples in HIV
Positive Patients.
 An Emerging Medical Device: Electrophoretic Sperm Separation.
 Magnetic Activated Cell Sorting of Human Spermatozoa.
 Intrauterine Insemination.
 Conventional IVF Insemination.
 Intra
Cytoplasmic Sperm Injection.
 ntracytoplasmic Morphologically Selected Sperm Injection.
 Intracytoplasmic Morphologically Selected Sperm Injection.
 Oocyte Activation Deficiency and Advances to Overcome.
 Hydraulic Manipulators for ICSI.
 Research Instruments Micromanipulation Systems .
 Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.
Oocyte Treatment and Preparation for Microinjection.
 Piezo
ICSI.
 Traditional Embryo Morphology Evaluation: From the Zygote to the Blastocyst Stage.
 Predicting Embryo Implantation Potential Using Video Monitoring By the Embryoscope Time
Lapse System.
 Predicting Embryo Developmental Potential and Viability Using Automated Time
Lapse Analysis.
 Proteomics and Metabolomics.
 Amino Acid Turnover as a Biomarker of Embryo Viability.
 Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology.
 Assisted Hatching of Human Embryos for Successful Implantation.
 Polar Body Biopsy.
 Cleavage
Stage Embryo Biopsy.
 Current and Novel Methods for Chromosome Testing.
Embryo Biopsy forPGD: Current Perspective.
 Sperm Cryopreservation.
 Technologies of Cryoprotectant
free Vitrification of Human Spermatozoa: Asepticity as Criterionn of Effectiveness.
 Slow Freezing of Oocytes.
 Vitrification: Methods Contributing to Successful Cyopreservation Outcomes.
 Oocyte Vitrification and Current Clinical Applications.
 Slow Freezing of Embryos.
 Vitrification of Embryos.
 Ovarian Tissue Cryopreservation.
 Establishing and Managing Donor Oocyte Banking.
 Single Embryo Transfer.
 Embryo Transfer:  Techniques and Trouble shooting.
 G
CSF and GM
CSF: Clinical applications in reproductive medicine.
 The Freeze All Cycle: A New Paradigm Shift in ART.
 IVF Data Management:  From Clipboards to Smart Apps.
 Regulation of the ART Laboratory.
 Legislation in the UK.
 Regulation, Licensing, and Accreditation of the ART Laboratory in Europe.
 Regulation, Licensing, and Accreditation of the ART Laboratory in Brazil.
 Regulation, Licensing and Accreditation of ART Laboratories in Australia.
 Trouble Shooting In Ivf Laboratories.
 Benchmarking and Decision Making in the IVF Laboratory.
 Automation, Artificial Intelligence and innovations in the future of IVF.
 Assisted reproductive technologies to prevent transmission of mitochondrial DNA disease.
 Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.
 Embryo culture and phenotype of the offspring.
 Traceability in ART.
 The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.
 Cytoskeletal architecture of human oocytes with a focus on centrosomes and their significant role in fertilization.
 Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.
 Livestock Production via Micromanipulation.