Intestinal Differentiated Cells (eBook, PDF)

Methods and Protocols
Redaktion: Ordóñez-Morán, Paloma

• Format: PDF

Produktbeschreibung

This volume details the most up-to-date methods of intestinal differentiation program. Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal differentiated cancer cells. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Intestinal Differentiated Cells: Methods and Protocols aims to be comprehensive guide for researchers.

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Produktdetails

• Verlag: Springer New York
• Seitenzahl: 275
• Erscheinungstermin: 13. Juni 2023
• Englisch
• ISBN-13: 9781071630761
• Artikelnr.: 68257845

Inhaltsangabe

Differentiated epithelial cells of the gut.- Identification of differentiated intestinal epithelial cells using immunostaining and fluorescence microscopy.- Novel approach to measure Trans Epithelial Electrical Resistance in intestinal cells.- In vivo model for isolating epithelial cells of the anorectal transition zone.- In vitro and in vivo assays for testing retinoids effect on intestinal progenitors' lineage commitments.- TORNADO-seq: a protocol for high-throughput targeted RNA-seq based drug screening in organoids.- Epigenetic and transcriptional dynamics of Notch program in intestinal differentiation.- Defining anorectal transition zone heterogeneity using single-Cell RNA sequencing.- Directed differentiation of murine and human small intestinal organoids towards all mature lineages.- Modeling Notch activity and lineage decisions using intestinal organoids.- Generation of fetal Intestinal organoids and their maturation into adultintestinal cells.- Visualization of differentiated cells in 3D- and 2D-intestinal organoid cultures.- In-vitro culture and histological evaluation of 3D organotypic cultures.- Fluorescence intensity and fluorescence lifetime imaging (FLIM) microscopies of cell differentiation in the small intestinal organoids using cholera toxin.- In vitro morphogenesis and differentiation of human intestinal epithelium in a gut-on-a-chip.- Co-culturing human intestinal enteroid monolayers with innate immune cells.- Measuring cell differentiation in colorectal cancer.- Intestinal cell differentiation and phenotype in 2D and 3D cell culture models.- Confocal laser scanning imaging of cell junctions in human colon cancer cells.- Automated quantitative analysis of shape features in human epithelial monolayers and spheroids generated from colorectal cancer cells.