Ion Channels (eBook, PDF)

Methods and Protocols
Redaktion: Stockand, James D.; Shapiro, Mark S.

• Format: PDF

Produktbeschreibung

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 216
• Erscheinungstermin: 4. Februar 2008
• Englisch
• ISBN-13: 9781597450959
• Artikelnr.: 64700609

Inhaltsangabe

Methods for Exogenous Expression of Ion Channels in Cells.- Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System.- Overexpression of Proteins in Neurons Using Replication-Deficient Virus.- Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System.- Methods for Studying Channel Structure-function.- Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels.- Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System.- Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis.- Methods for Studying Channel Regulation and Physiological Function.- Probing the Effects of Phosphoinositides on Ion Channels.- Epithelial Sodium Channel in Planar Lipid Bilayers.- A Simple In Vivo Method for Assessing Changes of Membrane-Bound Ion Channel Density in Xenopus Oocytes.- Preparation of Cortical Brain Slices for Electrophysiological Recording.- Juxtacellular Labeling and Chemical Phenotyping of Extracellularly Recorded Neurons In Vivo.- Carbon Fiber Amperometry in the Study of Ion Channels and Secretion.- Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology.- Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K+ Uptake-Deficient Escherichia coli.- KCNQ1 K+ Channel-Mediated Cardiac Channelopathies.- Tissue-Specific Transgenic and Knockout Mice.