Isolation and Purification of Proteins (eBook, PDF)

Redaktion: Hatti-Kaul, Rajni; Mattiasson, Bo

• Format: PDF

Produktbeschreibung

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

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Produktdetails

• Verlag: Taylor & Francis
• Seitenzahl: 676
• Erscheinungstermin: 5. Februar 2003
• Englisch
• ISBN-13: 9780203911686
• Artikelnr.: 38166829

Autorenporträt

Rajni Hatti-Kaul, Bo Mattiasson

Inhaltsangabe

Resolution technique for protein separation
selectivity in affinity chromatography
genetic approaches to facilitate purification
solid-liquid separation
membranes for protein isolation and purification
precipitation of proteins - non-specific and specific
protein crystallization for large-scale bioseparation
extraction for rapid protein isolation
adsorption as an initial step for the capture of proteins
fast chromatography of proteins
novel approaches to the chromatography of proteins
scale up and commercial production of recombinant proteins
process monitoring in downstream processing
protein formulation
regulatory requirements
cleaning and sanitization issues in protein purification.