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Inhaltsangabe
1 Introduction.- 1.1 The need for this book - an active extract, what happens next?.- 1.2 Why is it important to find out the nature of the active components?.- 1.3 Green chemicals - the renewed interest in natural products as sources of bioactive compounds.- 1.4 What are we doing?.- 1.5 Bibliography.- 2 Important concepts.- 2.1 Introduction.- Polarity.- 2.3 Dilution and concentration factors.- 2.4 Synergy.- 2.5 Deterioration, decomposition and storage.- 2.6 Biological variation.- 2.7 Authentication of material.- 2.8 Dereplication.- 3 Methods for extraction and sample clean-up.- 3.1 Introduction.- 3.2 Factors to be considered in selecting an extraction method.- 3.3 General extraction methods.- 3.4 Extraction methods for specific phytochemical groups.- 3.5 Clean-up techniques to remove unwanted substances.- 3.6 Bibliography.- 4 Crude fractionation procedures.- 4.1 Introduction.- 4.2 Choice of fractionation method.- 4.3 Common fractionation methods.- 4.4 Bibliography.- 5 Chromatographic fractionation procedures.- 5.1 Introduction.- 5.2 The principles of chromatography.- 5.3 Physico-chemical processes taking place in chromatography.- 5.4 Bibliography.- 6 Chromatographic procedures using columns and liquid-liquid systems.- 6.1 Column chromatography.- 6.2 Liquid-liquid partition schemes.- 6.3 Bibliography.- 7 Layer chromatography.- 7.1 Layer chromatography.- 7.2 Preparative thin-layer chromatography and paper chromatography.- 7.3 Bibliography.- 8 Analysis of crude extracts, fractions and isolated compounds.- 8.1 Introduction.- 8.2 Qualitative analysis of mixtures and isolates.- 8.3 Quantitative analytical methods.- 8.4 Biological assays.- 8.5 Bibliography.- 9 General methods.- 9.1 Basic techniques in handling extracts.- 9.2 Isolation of active compounds.- 9.3Chromatographic procedures.- 9.4 Basic techniques in thin-layer chromatography.- 9.5 Bibliography.- 10 Practical exercises in chemical screening and fractionation of crude extracts from plant materials.- 10.1 Chemical screening.- 10.2 Exercises in extraction, clean-up, fractionation and Chromatographicmethods.- Appendix A Recipes for chemical test reagents.
1 Introduction.- 1.1 The need for this book - an active extract, what happens next?.- 1.2 Why is it important to find out the nature of the active components?.- 1.3 Green chemicals - the renewed interest in natural products as sources of bioactive compounds.- 1.4 What are we doing?.- 1.5 Bibliography.- 2 Important concepts.- 2.1 Introduction.- Polarity.- 2.3 Dilution and concentration factors.- 2.4 Synergy.- 2.5 Deterioration, decomposition and storage.- 2.6 Biological variation.- 2.7 Authentication of material.- 2.8 Dereplication.- 3 Methods for extraction and sample clean-up.- 3.1 Introduction.- 3.2 Factors to be considered in selecting an extraction method.- 3.3 General extraction methods.- 3.4 Extraction methods for specific phytochemical groups.- 3.5 Clean-up techniques to remove unwanted substances.- 3.6 Bibliography.- 4 Crude fractionation procedures.- 4.1 Introduction.- 4.2 Choice of fractionation method.- 4.3 Common fractionation methods.- 4.4 Bibliography.- 5 Chromatographic fractionation procedures.- 5.1 Introduction.- 5.2 The principles of chromatography.- 5.3 Physico-chemical processes taking place in chromatography.- 5.4 Bibliography.- 6 Chromatographic procedures using columns and liquid-liquid systems.- 6.1 Column chromatography.- 6.2 Liquid-liquid partition schemes.- 6.3 Bibliography.- 7 Layer chromatography.- 7.1 Layer chromatography.- 7.2 Preparative thin-layer chromatography and paper chromatography.- 7.3 Bibliography.- 8 Analysis of crude extracts, fractions and isolated compounds.- 8.1 Introduction.- 8.2 Qualitative analysis of mixtures and isolates.- 8.3 Quantitative analytical methods.- 8.4 Biological assays.- 8.5 Bibliography.- 9 General methods.- 9.1 Basic techniques in handling extracts.- 9.2 Isolation of active compounds.- 9.3Chromatographic procedures.- 9.4 Basic techniques in thin-layer chromatography.- 9.5 Bibliography.- 10 Practical exercises in chemical screening and fractionation of crude extracts from plant materials.- 10.1 Chemical screening.- 10.2 Exercises in extraction, clean-up, fractionation and Chromatographicmethods.- Appendix A Recipes for chemical test reagents.
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