Macromolecular Crystallography Protocols, Volume 1 (eBook, PDF)

Preparation and Crystallization of Macromolecules
Redaktion: Doublie, Sylvie

• Format: PDF

Produktbeschreibung

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization. The volume concludes with a survey of available crystallographic software.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 280
• Erscheinungstermin: 4. Februar 2008
• Englisch
• ISBN-13: 9781597452090
• Artikelnr.: 64701677

Inhaltsangabe

A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag.- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project.- Baculoviral Expression of an Integral Membrane Protein for Structural Studies.- Protein Engineering.- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems.- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals.- Screening and Optimization Methods for Nonautomated Crystallization Laboratories.- Improving Marginal Crystals.- Optimization Techniques for Automation and High Throughput.- Three-Dimensional Crystallization of Membrane Proteins.- Crystallization of Protein-DNA Complexes.- Preparation and Crystallization of RNA.- Crystallization of RNA-Protein Complexes.