This text is essential for anyone working with cultures who wants to avoid the frustration of losing strains and needs to be able to devise and evaluate new strategies for preservation.
- Written by hands-on experts in their respective fields
- Contains helpful tables and protocols for preserving or maintaining cells, cultures and viruses
- Discusses means to preserve cells by freezing, lyophilization, drying, cyoprotection, spore storage, continuous propagation and subculturing when absolutely necessary, and others
- Gives information needed to test cultures for stable retention of important characteristics
- Gives principles needed to devise and evaluate preservation strategies for newly identified and newly engineered cells and organisms
- Lists culture sources for each class of organism
- Includes information for characterizing and monitoring recombinant organisms, especially important because of their propensity for genetic stability
- Discusses the history of the continually evolving field of culture preservation
- Examines the importance of genetically stable cultures as it relates to maintaining patent positions
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