Manual of Sperm Function Testing in Human Assisted Reproduction (eBook, PDF)

Redaktion: Agarwal, Ashok

• Format: PDF

Produktbeschreibung

Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men.

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Produktdetails

• Verlag: Cambridge University Press
• Erscheinungstermin: 15. April 2021
• Englisch
• ISBN-13: 9781108881890
• Artikelnr.: 66182180

Inhaltsangabe

Introduction R. John Aitken; 1. Standard semen analysis: manual semen
analysis Lars Björndahl; 2. Standard semen analysis: computer-assisted
semen analysis Liana Maree; 3. Standard semen analysis: home sperm testing
Manesh Panner Selvam Kumar, Christopher Douglas and Ashok Agarwal; 4.
Standard semen analysis: leukocytospermia Renata Finelli and Ralf Henkel;
5. Standard semen analysis: morphology Roelof Menkveld; 6. Sperm vitality:
eosin-nigrosin dye exclusion Rakesh Sharma and Ashok Agarwal; 7. Sperm
vitality: hypo-osmotic swelling test Alexandre Rouen and Rajasingam S.
Jeyendran; 8. Determination of mitochondrial membrane potential by flow
cytometry in human sperm cells Nicolas Germain, Nathalie Jouy, Carole
Marchetti and Philippe Marchetti; 9. Capacitation and acrosome reaction:
fluorescence techniques to determine acrosome reaction Raúl S. Sánchez
Gutiérrez, Fabiola Zambrano and Pamela Uribe; 10. Capacitation and acrosome
reaction: histochemical techniques to determine acrosome reaction Brett
Nixon, Shenae Café, Elizabeth Bromfield, Geoffry De Iuliis and Matthew Dun;
11. Zona binding: competitive sperm-binding assay De Yi Liu, Ying Zhong and
Zi-Na Wen; 12. Zona binding: hemizona assay Shubhadeep Roychoudhury and
Daniel Franken; 13. Oolemma binding: sperm penetration assay Anup Shah and
Kathleen Hwang; 14. Oxidative stress testing: direct tests Renata Finelli,
Manesh Kumar, Panner Selvam and Ashok Agarwal; 15. Oxidative stress
testing: indirect tests Rakesh Sharma, Kathy Robert and Ashok Agarwal; 16.
Chromatin condensation: aniline blue stain Jesse JP, Vidhu Dhawan and Rima
Dada; 17. Chromatin condensation: chromomycin A3 (CMA3) stain Sara
Marchiani, Lara Tamburrino, Francesca Benini, Sandra Pellegrini and
Elisabetta Baldi; 18. Sperm chromatin structure: Toluidine Blue Staining
Mohammed Nasr-Esfahani and Marziyeh Tavalaee; 19. DNA damage: TdT-mediated
dUTP nick-end-labelling (TUNEL) assay Rakesh Sharma, Concetta Iovine and
Ashok Agarwal; 20. DNA damage: sperm chromatin structure assay (SCSA) Don
Evenson; 21. DNA damage: COMET assay Luke Simon, Benjamin R. Emery and
Douglas T. Carrell; 22. DNA damage: halo sperm test Jaime Gosálvez and José
Luís Fernández; 23. DNA damage: fluorescent in-situ hybridization (FISH)
Sezgin Güne¿; 24. Clinical value of sperm function tests Ahmad Majzoub; 25.
Future developments: sperm proteomics Manesh Panner Selvam Kumar, Saradha
Baskaran and Ashok Agarwal; 26. Conclusion Ahmad Majzoub, Ralf Henkel and
Ashok Agarwal.