Mass Spectrometry Imaging of Small Molecules (eBook, PDF)

Redaktion: He, Lin

• Format: PDF

Produktbeschreibung

Mass Spectroscopy Imaging (MSI) has emerged as an enabling technique to provide insight into the molecular entities within cells, tissues and whole-body samples and to understand inherent complexities within biological metabolomes. In Mass Spectrometry Imaging of Small Molecules, experts in the MSI field present techniques for 2D and 3D visualization and quantification of a wide array of small molecular species present in biologically relevant samples. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification, and from data collection to visualization of small molecule mapping in complex samples. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Mass Spectrometry Imaging of Small Molecules aims to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Springer US
• Seitenzahl: 213
• Erscheinungstermin: 31. Oktober 2014
• Englisch
• ISBN-13: 9781493913572
• Artikelnr.: 64701321

Inhaltsangabe

Current Status and Future Prospects of Mass Spectrometry Imaging of Small Molecules.- Sample Preparation for 3D SIMS Chemical Imaging of Cells.- TOF-SIMS Imaging of Lipids on Rat Brain Sections.- MALDI-MS Assisted Molecular Imaging of Metabolites in Legume Plants.- MALDI Mass Spectrometry Imaging of Lipids and Primary Metabolites on Rat Brain Sections.- Multiplex MALDI-MS Imaging of Plant Metabolites Using a Hybrid MS System.- DESI Imaging of Small Molecules in Biological Tissues.- Desorption Electrospray Ionization Imaging of Small Organics on Mineral Surfaces.- Imaging of Plant Materials Using Indirect Desorption Electrospray Ionization Mass Spectrometry.- Imaging of Lipids and Metabolites Using Nanospray Desorption Electrospray Ionization Mass Spectrometry.- Electrospray Laser Desorption Ionization (ELDI) Mass Spectrometry for Molecular Imaging of Small Molecules on Tissues.- Automated Cell-By-Cell Tissue Imaging and Single Cell Analysis for Targeted Morphologies by Laser Ablation Electrospray Ionization Mass Spectrometry.- Laser Ablation Sample Transfer for Mass Spectrometry Imaging.- Nanostructure-Based Mass Spectrometry: High Sensitivity, Enzyme Activity Analysis and Metabolite Imaging.- Nanostructure-Initiator Mass Spectrometry (NIMS) for Molecular Mapping of Animal Tissues.- Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging.-Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imaging of Small Molecules.- Laser Desorption Postionization Mass Spectrometry Imaging of Biological Targets.- Data Processing and Analysis for Mass Spectrometry Imaging.

Rezensionen

"The purpose is to detail the rapidly maturing methods of metabolic imaging and to minimize technical challenges in adapting new technological platforms in biological research. These are quite worthy objectives that the book meets. The intended audience is life science researchers. The book also will be quite useful for students and scientists interested in learning more about mass spectrometry imaging of small molecules." (Omer Iqbal, Doody's Book Reviews, September, 2015)