Jean-Luc Popot

Membrane Proteins in Aqueous Solutions (eBook, PDF)

From Detergents to Amphipols

• Format: PDF

Produktbeschreibung

Membrane proteins represent about one third of the proteins encoded in a cell's genome, and, because of their key physiological roles, more than half of drug targets. Detergents are traditionally used to extract proteins from membranes in order to make them amenable to the tools of biochemistry and biophysics. However, detergent-solubilized proteins are generally unstable. This has led to the development of alternative, non-conventional surfactants, such as bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants, and specially designed amphipathic polymers called 'amphipols'. These novel tools, mainly developed over the past 20 years, are revolutionizing handling membrane proteins in vitro for basic and applied research, as well as for such biomedical applications as drug screening or vaccination.

This book, written by a specialist of membrane proteins and one of the creators of amphipols, describes the properties and usesof these novel molecules. It opens with general introductions on membrane proteins and their natural environment, detergents, the current status of membrane protein in vitro studies, a broad panorama of non-conventional surfactants and a discussion of their respective advantages and limitations, and the preparation and properties of amphipols and membrane protein/amphipol complexes. Topical chapters cover in vitro folding, cell-free synthesis and stabilization of membrane proteins, and such biophysical and biochemical applications as electron microscopy, Xray diffraction, NMR, optical spectroscopy, mass spectrometry, the whole range of solutions studies, proteomics, and such practical applications as membrane protein immobilization and drug screening and the use of amphipols in vivo for vaccination and drug delivery. Each topical chapter is introduced with a concise, up-to-date overview of how membrane proteins are currently studied using each individual technique, before offering an exhaustive coverage and in-depth discussion of the contribution of amphipols, and concluding with hands-on protocols written by everyday practitioners of each application.

In addition to a comprehensive coverage of the properties and uses of non-conventional surfactants, this book therefore also offers a concise, accessible introduction to membrane protein biochemistry and biophysics. It is meant to be used both in basic and applied research laboratories and as a teaching help.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Springer Nature Switzerland
• Seitenzahl: 708
• Erscheinungstermin: 8. Juni 2018
• Englisch
• ISBN-13: 9783319731483
• Artikelnr.: 56792996

Autorenporträt

Jean-Luc Popot, born 1948, studied biology, chemistry, and biophysics in Orléans and Orsay. In 1971, he took a permanent position with the Collège de France. In J.-P. Changeux's laboratory, at the Pasteur Institute, he applied electrophysiology and biophysical and biochemical approaches to functional and structural studies of the nicotinic acetylcholine receptor. In 1982, he joined D.M. Engelman at Yale University as a visiting scientist. His work at Yale, which bore principally on refolding bacteriorhodopsin from denatured fragments and studying the refolded structure crystallographically, led the two of them to propose, in 1990, an influential model for the folding of a-helical membrane proteins. In 1985, he joined the laboratory of P. Joliot at the Institut de Biologie Physico-Chimique (Paris). His group carried out neutron diffraction and model building work on bacteriorhodopsin and biochemical studies on Photosystem II and cytochrome b₆ f. In 1996, he became Research Director at the Centre National de la Recherche Scientifique and created his own laboratory, where the X-ray structure of the b₆ f was solved in 2003. In parallel, he pursued the development of sequence analysis approaches and, in collaboration with chemists and physical chemists, designed and validated non-conventional surfactants aimed at facilitating membrane protein solution studies, most notably amphipathic polymers ('amphipols') and fluorinated surfactants. He retired in 2013 and, along with his wife, splits his time between restoring an old house in Languedoc, reading, writing, hiking, photographing, cooking, and enjoying the company of kith and kin.

Inhaltsangabe

From the Contents: Membrane proteins: functions, structures, environments.- Taking membrane proteins out of their natural environment.- Alternatives to detergents for handling membrane proteins in aqueous solutions.- Chemical structure and physical-chemical properties of amphipols.

Kundenbewertungen

Bewertung schreiben Bewertung schreiben

Information zu Bewertungen

Zur Abgabe einer Bewertung ist die Anmeldung in Ihrem Kundenkonto notwendig. Die Authentizität der Kundenbewertungen wird von uns nicht überprüft.

Bewertung schreiben Bewertung schreiben