Metalloproteins (eBook, PDF)

Methods and Protocols
Redaktion: Fontecilla-Camps, Juan C.; Nicolet, Yvain

• Format: PDF

Produktbeschreibung

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 299
• Erscheinungstermin: 17. März 2014
• Englisch
• ISBN-13: 9781627037945
• Artikelnr.: 64560337

Inhaltsangabe

Advances in Research on Metalloproteins.- Purification of O2-Sensitive Metalloproteins.- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources.- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins.- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers.- Electrochemistry of Metalloproteins - Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1.- FTIR Spectroscopy of Metalloproteins.- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy.- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds.- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR).- Mössbauer Spectroscopy.- X-Ray Absorption Spectroscopy of Metalloproteins.- X-Ray Crystallographic Studies of Metalloproteins.- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds.- Density Functional Theory - Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.