Methods for Studying Nucleic Acid/Drug Interactions (eBook, PDF)

Redaktion: Wanunu, Meni; Tor, Yitzhak

• Format: PDF

Produktbeschreibung

This book describes emerging, non-conventional methods for probing the interactions of small molecules with nucleic acids as well as modern computational methods for assessment. By highlighting novel methods for nucleic acid/ligand interactions, the text presents drug developing companies with a survey of possible future techniques as well as their drawbacks and advantages with respect to common techniques. In addition, and perhaps more importantly, this book is designed to inspire young scientists to continue and advance these methods into fruition, especially in light of current capabilities for assay miniaturization and enhanced sensitivity using microfluidics and nanomaterials.

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Produktdetails

• Verlag: Taylor & Francis
• Seitenzahl: 392
• Erscheinungstermin: 19. April 2016
• Englisch
• ISBN-13: 9781439839744
• Artikelnr.: 57532785

Autorenporträt

Meni Wanunu completed his Ph.D. in 2005 at the Weizmann Institute of Science, where he specialized in supramolecular chemistry, self-assembly, and nanomaterials science. He then carried out a postdoctoral position at Boston University and a research associate position at the University of Pennsylvania, where he developed ultrasensitive synthetic nanopores for nucleic acid analysis at the single-molecule level. Currently, he is an Assistant Professor at the Department of Physics and the Department of Chemistry and Chemical Biology at Northeastern University, Boston. His research interests include developing chemical approaches for investigating biomolecular structure and behavior, nucleic acid mechanics and dynamics, and probing biological processes at the single-molecule level. Yitzhak Tor carried out his doctorate work at the Weizmann Institute of Science, earning his Ph.D. in 1990. After a postdoctoral stay at the California Institute of Technology (1990-1993), he took his first faculty position at the University of Chicago. In 1994, he moved to the University of California, San Diego, where he is currently a Professor of Chemistry and Biochemistry and the Traylor Scholar in Organic Chemistry. His research interests are diverse and include chemistry and biology of nucleic acids, the discovery of novel antiviral and antibacterial agents, as well as the development of cellular delivery agents and fluorescent probes. He is currently the Editor in Chief of Perspectives in Medicinal Chemistry (http://la-press.com/journal.php?journal_id=25) and Organic Chemistry Insights (http://www.la-press.com/organic-chemistry-insights-journal-j104). Away from chemistry, his interests are predominantly in music, playing, recording and producing his own instrumental CDs.

Inhaltsangabe

CLASSICAL TECHNIQUES: Using Spectroscopic Techniques to Examine Drug-DNA
Interactions. Probing DNA and RNA Interactions with Biogenic and Synthetic
Polyamines: Models and Biological Implications. EMERGING TECHNIQUES: Mass
Spectrometry-Based Techniques for Studying Nucleic Acid/Small Molecule
Interactions.
Real-Time Monitoring of Nucleic Acid Interactions with Biosensor-Surface
Plasmon Resonance. Studying Aptamer/Ligand Interactions Using Fluorescence
Correlation Spectroscopy. Studying Nucleic Acid - Drug Interactions at the
Single Molecule Level Using Optical Tweezers. Fluorescent Nucleoside
Analogues for Monitoring RNA-Drug Interactions. Atomic Force Microscopy
Investigation of DNA-Drug Interactions. Characterizing RNA-Ligand
Interactions Using 2-Dimensional Combinatorial Screening. EPR Spectroscopy
for the Study of RNA-Ligand Interactions. Electrochemical Approaches to the
Study of DNA-Drug Interactions. Nanopore Ion Microscope for Detecting
Nucleic Acid/Drug Interactions. A Primer for Relaxation Kinetic
Measurements. DNA-Drug Interactions: A Theoretical Perspective.
Computational Studies of RNA Dynamics and RNA-Ligand Interactions.
All chapters include case studies, background and basics, prospects and
outlook, conclusions, and references