Methods in Human Cytogenetics (eBook, PDF)

Redaktion: Schwarzacher, H. G.; Wolf, U.

• Format: PDF

Produktbeschreibung

This volume was originally intended to be an English translation of the book MetllOden in der medizinischen Cytogenetik, published in 1970. Just about then, however, a number of new techniques were introduced in human cytogenetics and soon acquired the utmost importance, parti cularly in clinical diagnosis, so that the English edition had to be con siderably enlarged. As a result, there are now twelve chapters instead of eight, and two additional authors have been called upon, Dr. KRONE and Dr. SCHNEDL. In addition to the up-to-date presentation of con ventional methods of cell culture and techniques for the preparation and identification of human chromosomes, this text covers the various tech niques of producing banding patterns and applying them in chromo some identification. Further, it deals with the culture of amniotic fluid cells and gives instructions for handling tissue-culture cells for bio chemical analysis; it thus meets the ever-increasing requirements of a modern cell-culture laboratory. To paraphrase the aims of this book, we quote part of the preface to the German edition: "It was intended to collect the various methods so as to make them accessible for laboratory use. Furthermore, it is hoped that the reader faced with current research problems will be stimulated to modify and supplement the techniques described, instead of merely applying them automatically. In a rapidly developing field, some methods are still preliminary, and no final presentation seems possible.

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Produktdetails

• Verlag: Springer Berlin Heidelberg
• Seitenzahl: 298
• Erscheinungstermin: 6. Dezember 2012
• Englisch
• ISBN-13: 9783642657870
• Artikelnr.: 53390438

Inhaltsangabe

I Cell Cultures from Blood and Bone Marrow.- 1. Introduction.- 2. Bone Marrow.- 3. Culture of Peripheral Blood Cells.- 4. Phytohemagglutinin: Properties and Mode of Action.- Appendix: Laboratory Procedures.- References.- II Cell Cultures from Tissue Expiants.- 1. Introduction.- 2. Areas of Application.- 3. Material.- 4. Introduction to Methods.- 5. Techniques for Collecting Tissue.- 6. Setting up Tissue Expiants and Culturing Cells.- 7. Harvest of Cultures for Chromosome Preparation.- 8. Long Term Preservation of Tissue Culture Cells.- Appendix I: Summary of Some of the Methods.- Appendix II: Catalog of Some Reagents, Media and Culture Vessels.- References.- III Culture and Preparation of Cells from Amniotic Fluid.- 1. Introduction.- 2. Collecting and Transportation of Amniotic Fluid.- 3. X- and Y-Chromatin.- 4. Cell Culture.- Appendix: Protocol for Amniotic Fluid Cultures.- References.- IV Preparation of Metaphase Chromosomes.- 1. Introduction.- 2. Preparation of Cell Suspensions.- 3. Direct Preparation from Monolayer Cultures.- 4. Direct Preparation of Embryonic Tissue.- 5. Staining.- Appendix: Laboratory Guide.- References.- V Fluorescence Microscopy of Chromosomes and Interphase Nuclei.- 1. Introduction.- 2. Fluorescent Dyes for Chromosomes and Cell Nucle.- 3. Other Fluorochromes.- 4. Technique of Fluorescence Microscopy.- 5. Analysis of Preparations.- Appendix 1: Staining with Quinacrine-Dihydrochloride and Quinacrine-Mustard.- Appendix 2: Suppliers of Quinacrine Stains.- Appendix 3: Phosphate Buffer Solution (Sörensen).- References.- VI Banding Patterns in Human Chromosomes Visualized by Giemsa Staining after Various Pretreatments.- 1. Introduction.- 2. General Comments on the Different Methods.- 3. Methods for Demonstrating the Centromeric Heterochromatin.- 4.Methods for Demonstrating Banding Patterns over the Entire Chromosome.- Appendix: Laboratory Procedures.- References.- VII Autoradiography of Human Chromosomes with 3H-Thymidine.- 1. Introduction.- 2. Theoretical Basis.- 3. Techniques.- Appendix: Summary of the Procedure.- References.- VIII The Human Karyotype Analysis of Chromosomes in Mitosis and Evaluation of Cytogenetic Data.- 1. Introduction.- 2. Chromosomes at Normal Metaphase.- 3. The Standard Karyotype.- 4. Identification of Individual Chromosomes by Special Methods.- 5. Variability of the Karyotype.- 6. Evaluation of Cytogenetic Data.- 7. Nomenclature.- Appendix: Tables.- References.- IX Analysis of Interphase Nuclei.- 1. Introduction.- 2. Y-Chromatin.- 3. X-Chromatin.- 4. Techniques for Preparing Interphase Nuclei.- 5. Staining.- 6. Evaluation of the Preparations.- Appendix: Laboratory Procedures.- References.- X The Diagnosis of X-Chromatin by the Leukocyte Test.- 1. Introduction.- 2. Methods.- 3. Evaluation.- References.- XI Chromosomes in Meiosis.- 1. Introduction.- 2. Review of the Meiotic Process in Man.- 3. Preparations for Meiotic Prophase Figures.- 4. Preparations for Meiotic Metaphase Figures.- 5. Interpreting the Findings.- 6. Summary.- References.- XII Marginal Notes on the Handling of Tissue Culture Cells for Biochemical Analysis.- 1. Introduction.- 2. Selected General Notes.- 3. Discussion of Some Procedures.- 4. Presentation of Results.- References.